LOCUS       sequence-66879-e         960 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Spitzer JI, Ugras S, Runge S, Decarolis P, Antonescu C, Tuschl T, 
            Singer S
  TITLE     mRNA and protein levels of FUS, EWSR1, and TAF15 are upregulated in 
            liposarcoma.
  JOURNAL   Genes Chromosomes Cancer. 2011 May . 50(5):338-47.
  PUBMED    21344536
REFERENCE   2  (bases 1 to 960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes 
            Chromosomes Cancer. 2011 May . 50(5):338-47."
COMMENT     Sequence Label: pFRT_TO_DESTFLAGHA
FEATURES             Location/Qualifiers
     source          1..960
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(132..256)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             309..350
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(430..447)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    436..660
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(793..812)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ggtctccgtt atcggggaag aagtggctga tctcagccac cgcgaaaatg acatcaaaaa
       61 cgccattaac ctgatgttct ggggaatata aatgtcaggc tcccttatac acagccagtc
      121 tgcaggtcga ccatagtgac tggatatgtt gtgttttaca gtattatgta gtctgttttt
      181 tatgcaaaat ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcagctt
      241 tcttgtacaa agtggttgat atccagcaca gtggcggccg ctcaagtcta gagggcccgc
      301 ggttcgaagg taagcctatc cctaaccctc tcctcggtct cgattctacg cgtacctgat
      361 ccgcggccgc atagataact gatccagtgt gcctcgagtc tagagggccc gtttaaaccc
      421 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
      481 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
      541 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
      601 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
      661 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
      721 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
      781 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
      841 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
      901 tcgaccccaa aaaacttgat tagggtgatg gttcacgtac ctagaagttc ctattccgaa
//
