LOCUS       sequence-66888-e         942 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 942)
  TITLE     Craig lab unpublished plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pEGFPC3/GgVcl 884-1066 (Vt)
FEATURES             Location/Qualifiers
     source          1..942
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(651..667)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(652..668)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    809..930
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(846..865)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     900..919
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 gacgagctgt acaagtactc agatctcgag ctcaagcttc gaattctgca ggaattccca
       61 gagcagaaag caggagaagc tattaatcag cccatgatga tggctgctag gcagttgcat
      121 gacgaggctc ggaaatggtc tagcaagggt aacgacatca ttgctgctgc taaacgaatg
      181 gcgctgctca tggctgagat gtcacgcctg gtgcgaggag gcagcggaaa caagcgtgcc
      241 ctcatccagt gtgcaaaaga tattgctaag gcatcggatg aagtcactcg attggccaaa
      301 gaggtggcca agcaatgtac tgataaacgc attagaacaa acctcttaca ggtctgtgag
      361 cgaatcccaa ccatcagcac gcagctcaaa attctttcca cagtgaaagc taccatgctg
      421 ggcaggacta acatcagcga tgaagaatca gaacaggcaa ctgagatgtt ggttcataac
      481 gcccagaacc tcatgcagtc tgtgaaggaa actgtgagag aagctgaagc agcatccatt
      541 aagataagaa cagatgccgg attcactctg cgctgggtca gaaagacccc atggtatcag
      601 taaacaccac ataagcattg tcttctgtcg gaattcgata tcaagcttat cgataccgtc
      661 gacctcgagg gggggcccgg gatccaccgg atctagataa ctgatcataa tcagccatac
      721 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
      781 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
      841 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
      901 tggtttgtcc aaactcatca atgtatctta acgcgtaaat tg
//
