LOCUS       sequence-66920-e         917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Maier CJ, Maier RH, Hintner H, Bauer JW, Onder K
  TITLE     Coupled yeast 2-hybrid-mammalian 2-hybrid reading-frame-independent 
            and site-specific recombinational cloning vector system.
  JOURNAL   Assay Drug Dev Technol. 2010 Oct;8(5):625-9. doi: 
            10.1089/adt.2009.0266. Epub 2010 Jun 1.
  PUBMED    20515414
REFERENCE   2  (bases 1 to 917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1089/adt.2009.0266"; journalName: "Assay Drug Dev Technol"; 
            date: "2010-10"; volume: "8"; issue: "5"; pages: "625-9"
COMMENT     Sequence Label: pMBD-Gate2
FEATURES             Location/Qualifiers
     source          1..917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    49..173
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(198..203,204..221,222..228)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(348..367)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 gtatcgccgg aattcccggg gatccgtcga cttgacgcgt tgatatcaac aagtttgtac
       61 aaaaaagctg aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg
      121 cataaaaaac agactacata atactgtaaa acacaacata tccagtcact atggcggccg
      181 cattaggcac cccaggcttt acactttatg cttccggctc gtataatgtg tggattttga
      241 gttaggatcc gtcgagattt tcaggagcta aggaagctaa aatggagaaa aaaatcactg
      301 gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag gcatttcagt
      361 cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc tttttaaaga
      421 ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt gcccgcctga
      481 tgaatgctca tccggaattc cgtatggcaa tgaaagacgg tgagctggtg atatgggata
      541 gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca tcgctctgga
      601 gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat gtggcgtgtt
      661 acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt ttcgtctcag
      721 ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg gacaacttct
      781 tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc
      841 tggcgattca ggttcatcat gccgtttgtg atggcttcca tgtcggcaga atgcttaatg
      901 aattacaaca gtactgc
//
