LOCUS       sequence-67090-e         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Taelman VF, Dobrowolski R, Plouhinec JL, Fuentealba LC, Vorwald PP, 
            Gumper I, Sabatini DD, De Robertis EM
  TITLE     Wnt signaling requires sequestration of glycogen synthase kinase 3 
            inside multivesicular endosomes.
  JOURNAL   Cell. 2010 Dec 23. 143(7):1136-48.
  PUBMED    21183076
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2010 
            Dec 23. 143(7):1136-48."
COMMENT     Sequence Label: pCS2 GFP-GSK3-MAPK-E3mut
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    79..213
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(116..135)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     170..189
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(254..277)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(418..439)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     767..786
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 cgccgcggcc gcgaattaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
       61 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
      121 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
      181 aaactcatca atgtatctta tcatgtctgg atctacgtaa tacgactcac tatagttcta
      241 gaggctcgag ttacttgtca tcgtcgtcct tgtagtcggc agcggcagcg gcagcggcag
      301 cggcagcggc agcaggagaa gcaggagcag aagcggcagc agaagcggca gcagaagcgg
      361 cagctgaatt cgattcttgt acagctcgtc catgccgaga gtgatcccgg cggcggtcac
      421 gaactccagc aggaccatgt gatcgcgctt ctcgttgggg tctttgctca gggcggactg
      481 ggtgctcagg tagtggttgt cgggcagcag cacggggccg tcgccgatgg gggtgttctg
      541 ctggtagtgg tcggcgagct gcacgctgcc gtcctcgatg ttgtggcgga tcttgaagtt
      601 caccttgatg ccgttcttct gcttgtcggc catgatatag acgttgtggc tgttgtagtt
      661 gtactccagc ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc ccttcagctc
      721 gatgcggttc accagggtgt cgccctcgaa cttcacctcg gcgcgggtct tgtagttgcc
      781 gtcgtccttg aagaagatgg tgcgctcctg gacgtagcct tcgggcatgg cggacttgaa
//
