LOCUS       sequence-6720-ex        7620 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7620)
  AUTHORS   Malagon F, Tong AH, Shafer BK, Strathern JN
  TITLE     Genetic interactions of DST1 in Saccharomyces cerevisiae suggest a 
            role of TFIIS in the initiation-elongation transition.
  JOURNAL   Genetics 2004 Mar;166(3):1215-27.
  PUBMED    15082542
REFERENCE   2  (bases 1 to 7620)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics"; 
            date: "2004-03"; volume: "166(3)"; pages: "1215-27"
COMMENT     Sequence Label: YEplac181-SUA7
FEATURES             Location/Qualifiers
     source          1..7620
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(142..147,148..165,166..172)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     185..207
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     204..220
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2169..2186)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2169..2185)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2178..2200)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2622..3968
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     promoter        complement(5210..5608)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     primer_bind     complement(5628..5646)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5714..5818
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5819..5887,5888..6679)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6037..6056)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6850..7438
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7339..7358
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7592..7609
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc
      241 atgcctgcat atttcaaata acttgtttag ctttcatcct ttcgaatacc tccttcaata
      301 aaaaaaaaaa gaaaaatctt gttaaattat aaagtgttca ttctattttt ttgaattgta
      361 aaataacaaa ataaatattc gattgaactg ccatggaaaa ggttctaccc tctaacacga
      421 gtacccgtgc ttcttgttcc tataatttac tgttttatca cttcattatt tcttttcaac
      481 gcccggtaag ttatccaaag acactacacc attagcaata agctgcggat ccacaagctt
      541 gtctctatgc tcataaagta tcttgtaacc agatttgatg gtaccttcag taacttgcaa
      601 ggtttggccc actttcgctg cggtaatagg gatctgaaag agcaggatgt ttagatagat
      661 ggaaacaaca gcgatagtaa taggggattt acctgcaatt tctttgatct ctttacattt
      721 ctttgcggta tattcagcag aagtagtgac ttgcatcggt aagcccagat gcgaacaaaa
      781 cctgggtata taagttaggt tttgtgcacc actcatatta tcggtatcaa tcttcaagaa
      841 accatcttcg ctcttgcctc ttaaaatgtt cttcattatg tttaaagttt taccaaactc
      901 tttagttttg acatggatta atgactggat ttctttaaaa gtccttgcca cttcagcacg
      961 tctgcaacca atcagtatgg atgcagccat tatactctcc attgatttcc ccttcaaagt
     1021 tttttcatcg tggcaaagtt tatatgcttc cttggcacaa tctttcacaa tcttcggtaa
     1081 ttcagcagca tcacatagca tggtaatctt agcaaatgca gcttgtactt cattatcctt
     1141 tttatccatc acattttttc cttgtgcctt attcagttct ttggtgaatc tcatatccgt
     1201 ggtttcaccc tttccgatcc ttgtagatag gttattccca tctaaaagag gatttgaagc
     1261 ctcaccaaca cgacttgggt catcaccgtt gtgatcatca tttgaaaacg ttctccactc
     1321 cgacctggtg tcaaccagtt tatctgatag tactagacca catagagcac atacaacatc
     1381 cccttcacta aatctttcaa cgatttttgg tgggtagact ttgcactccg gacacgtcag
     1441 cacaatattc agattaggac cccttcttcc tgctctttta tctatgctct ccctagtcat
     1501 cattctgcgt atctacttct ccccttctct atcactggat cgtccacacc agcgtctatt
     1561 aagttgtctt tataagtgat gattattctc ttcacttttt tttaactaaa tttataacaa
     1621 aatttttcga tttttaagca aatattgaga cgaaacggtg atgagtaaga cagaaataca
     1681 gagatactaa gctataaact actatttaag cacgtgcata aattgggtgt gatgtgcagt
     1741 caaggcgagc gtcgattaca atgggcgtag aacacatgag aggctttagt aatgagataa
     1801 tcctgaagag aacgttgact ttatccgatt tcaccttacg ttaccataaa aggggcatca
     1861 cagctttgca ggtcatcaaa gcaccgtcag tttcgaatgt gccagtcttg ttatccggtg
     1921 ataactacgg ttatttcgtt atgtgggacc tggtgaccaa gagaccgatt acacatatag
     1981 aaatcgaggg caactcgcac attatagcgt tttggtgggt ggaaactacc aatgtgctgt
     2041 acattctttc gaaagactcc atgctgcgaa tttttgagct tgattcatct acacaactat
     2101 ctatagattt agttagaaag cttagccaag ccaataaaac ggatcattag ggtaccgagc
     2161 tcgaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     2221 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     2281 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat
     2341 tttctcctta cgcatctgtg cggtatttca caccgcatat atcggatcgt acttgttacc
     2401 catcattgaa ttttgaacat ccgaacctgg gagttttccc tgaaacagat agtatatttg
     2461 aacctgtata ataatatata gtctagcgct ttacggaaga caatgtatgt atttcggttc
     2521 ctggagaaac tattgcatct attgcatagg taatcttgca cgtcgcatcc ccggttcatt
     2581 ttctgcgttt ccatcttgca cttcaatagc atatctttgt taacgaagca tctgtgcttc
     2641 attttgtaga acaaaaatgc aacgcgagag cgctaatttt tcaaacaaag aatctgagct
     2701 gcatttttac agaacagaaa tgcaacgcga aagcgctatt ttaccaacga agaatctgtg
     2761 cttcattttt gtaaaacaaa aatgcaacgc gagagcgcta atttttcaaa caaagaatct
     2821 gagctgcatt tttacagaac agaaatgcaa cgcgagagcg ctattttacc aacaaagaat
     2881 ctatacttct tttttgttct acaaaaatgc atcccgagag cgctattttt ctaacaaagc
     2941 atcttagatt actttttttc tcctttgtgc gctctataat gcagtctctt gataactttt
     3001 tgcactgtag gtccgttaag gttagaagaa ggctactttg gtgtctattt tctcttccat
     3061 aaaaaaagcc tgactccact tcccgcgttt actgattact agcgaagctg cgggtgcatt
     3121 ttttcaagat aaaggcatcc ccgattatat tctataccga tgtggattgc gcatactttg
     3181 tgaacagaaa gtgatagcgt tgatgattct tcattggtca gaaaattatg aacggtttct
     3241 tctattttgt ctctatatac tacgtatagg aaatgtttac attttcgtat tgttttcgat
     3301 tcactctatg aatagttctt actacaattt ttttgtctaa agagtaatac tagagataaa
     3361 cataaaaaat gtagaggtcg agtttagatg caagttcaag gagcgaaagg tggatgggta
     3421 ggttatatag ggatatagca cagagatata tagcaaagag atacttttga gcaatgtttg
     3481 tggaagcggt attcgcaata ttttagtagc tcgttacagt ccggtgcgtt tttggttttt
     3541 tgaaagtgcg tcttcagagc gcttttggtt ttcaaaagcg ctctgaagtt cctatacttt
     3601 ctagctagag aataggaact tcggaatagg aacttcaaag cgtttccgaa aacgagcgct
     3661 tccgaaaatg caacgcgagc tgcgcacata cagctcactg ttcacgtcgc acctatatct
     3721 gcgtgttgcc tgtatatata tatacatgag aagaacggca tagtgcgtgt ttatgcttaa
     3781 atgcgtactt atatgcgtct atttatgtag gatgaaaggt agtctagtac ctcctgtgat
     3841 attatcccat tccatgcggg gtatcgtatg cttccttcag cactaccctt tagctgttct
     3901 atatgctgcc actcctcaat tggattagtc tcatccttca atgctatcat ttcctttgat
     3961 attggatcga tccgatgata agctgtcaaa catgagaatt aattctaccc tatgaacata
     4021 ttccattttg taatttcgtg tcgtttctat tatgaatttc atttataaag tttatgtaca
     4081 aatatcataa aaaaagagaa tctttttaag caaggatttt cttaacttct tcggcgacag
     4141 catcaccgac ttcggtggta ctgttggaac cacctaaatc accagttctg atacctgcat
     4201 ccaaaacctt tttaactgca tcttcaatgg ccttaccttc ttcaggcaag ttcaatgaca
     4261 atttcaacat cattgcagca gacaagatag tggcgatagg gtcaacctta ttctttggca
     4321 aatctggagc agaaccgtgg catggttcgt acaaaccaaa tgcggtgttc ttgtctggca
     4381 aagaggccaa ggacgcagat ggcaacaaac ccaaggaacc tgggataacg gaggcttcat
     4441 cggagatgat atcaccaaac atgttgctgg tgattataat accatttagg tgggttgggt
     4501 tcttaactag gatcatggcg gcagaatcaa tcaattgatg ttgaaccttc aatgtaggaa
     4561 attcgttctt gatggtttcc tccacagttt ttctccataa tcttgaagag gccaaaacat
     4621 tagctttatc caaggaccaa ataggcaatg gtggctcatg ttgtagggcc atgaaagcgg
     4681 ccattcttgt gattctttgc acttctggaa cggtgtattg ttcactatcc caagcgacac
     4741 catcaccatc gtcttccttt ctcttaccaa agtaaatacc tcccactaat tctctgacaa
     4801 caacgaagtc agtaccttta gcaaattgtg gcttgattgg agataagtct aaaagagagt
     4861 cggatgcaaa gttacatggt cttaagttgg cgtacaattg aagttcttta cggattttta
     4921 gtaaaccttg ttcaggtcta acactacctg taccccattt aggaccaccc acagcaccta
     4981 acaaaacggc atcaaccttc ttggaggctt ccagcgcctc atctggaagt gggacacctg
     5041 tagcatcgat agcagcacca ccaattaaat gattttcgaa atcgaacttg acattggaac
     5101 gaacatcaga aatagcttta agaaccttaa tggcttcggc tgtgatcttg accaacgtgg
     5161 tcacctggca aaacgacgat cttcttaggg gcagacatag gggcagacat tagaatgtat
     5221 atccttgaaa tatatatata tattgctgaa atgtaaaagg taagaaaagt tagaataaga
     5281 cgattgctaa ccacctattg gaaaaaacaa taggtcctta aataatattg tcaacttcaa
     5341 gtattgtgat gcaagcattt agtcatgaac gcttctctat tctatatgaa aagccggttc
     5401 cggcctctca cctttccttt ttctcccaat ttttcagttg aaaaaggtat atgcgcggcg
     5461 acctctgaaa ttaacaaaaa atttccagtc atcgaatttg tctgtgcgat agcgcccctg
     5521 tgtgttctcg ttatgttgag gaaaaaaata atggttgcta agagattcga actcttgcat
     5581 cttacgatac ctgagtattc ccacagttaa ttcttgaaga cgaaagggcc tcgtgatacg
     5641 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     5701 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     5761 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     5821 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     5881 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     5941 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     6001 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     6061 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     6121 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     6181 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     6241 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     6301 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     6361 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     6421 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     6481 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     6541 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     6601 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     6661 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     6721 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     6781 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     6841 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     6901 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     6961 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     7021 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     7081 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     7141 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     7201 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     7261 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     7321 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     7381 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     7441 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     7501 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     7561 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
//
