LOCUS       sequence-67402-e         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  TITLE     Murray Lab pBEST plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pBEST-p15a-OR2-OR1-Pr-UTR1-sigma28-T500
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             170..190
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 cgatatccgc ctgatgcgtg aacgtgacgg acgtaaccac cgcgacatgt gtgtgctgtt
       61 ccgctgggca tgccaggaca acttctggtc cggtaacgtg ctgagctaac accgtgcgtg
      121 ttgacaattt tacctctggc ggtgataatg gttgcagcta gcaataattt tgtttaactt
      181 taagaaggag gatccaaatg aattcactct ataccgctga aggtgtaatg gataaacact
      241 cgctgtggca gcgttatgtc ccgctggtgc gtcacgaagc attgcgcctg caggttcgac
      301 tgcccgcgag cgtggaactt gacgatctgc tacaggcggg cggcattggg ttacttaatg
      361 ccgtcgaacg ctatgacgcc ctacaaggaa cggcatttac aacttacgca gtgcagcgta
      421 tccgtggcgc tatgctggat gaacttcgca gccgtgactg ggtgccgcgc agcgtgcgac
      481 gcaacgcgcg tgaagtggca caggcaatag ggcaactgga gcaggaactt ggccgcaacg
      541 ccacggaaac tgaggtagcg gaacgtttag ggatcgatat tgccgattat cgccaaatgt
      601 tgctcgacac caataacagc cagctcttct cctacgatga gtggcgcgaa gagcacggcg
      661 atagcatcga actggttact gatgatcatc agcgagaaaa cccgctacaa caactactgg
      721 acagtaatct gcgccagcgg gtgatggaag ccatcgaaac gttgccggag cgcgaaaaac
      781 tggtattaac cctctattac caggaagagc tgaatctcaa agagattggc gcggtgctg
//
