LOCUS       sequence-67433-e         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses mammalian codon optimized Cas9 nuclease with C-term 3X 
            FLAG from CMV and T7 promoters.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Joung JK
  TITLE     Joung lab unpublished CRISPR plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: JDS246
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      62..71
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gtgaaccgtc agatccgcta gagatccgcg gccgctaata cgactcacta tagggagagc
       61 cgccaccatg gataaaaagt attctattgg tttagacatc ggcactaatt ccgttggatg
      121 ggctgtcata accgatgaat acaaagtacc ttcaaagaaa tttaaggtgt tggggaacac
      181 agaccgtcat tcgattaaaa agaatcttat cggtgccctc ctattcgata gtggcgaaac
      241 ggcagaggcg actcgcctga aacgaaccgc tcggagaagg tatacacgtc gcaagaaccg
      301 aatatgttac ttacaagaaa tttttagcaa tgagatggcc aaagttgacg attctttctt
      361 tcaccgtttg gaagagtcct tccttgtcga agaggacaag aaacatgaac ggcaccccat
      421 ctttggaaac atagtagatg aggtggcata tcatgaaaag tacccaacga tttatcacct
      481 cagaaaaaag ctagttgact caactgataa agcggacctg aggttaatct acttggctct
      541 tgcccatatg ataaagttcc gtgggcactt tctcattgag ggtgatctaa atccggacaa
      601 ctcggatgtc gacaaactgt tcatccagtt agtacaaacc tataatcagt tgtttgaaga
      661 gaaccctata aatgcaagtg gcgtggatgc gaaggctatt cttagcgccc gcctctctaa
      721 atcccgacgg ctagaaaacc tgatcgcaca attacccgga gagaagaaaa atgggttgtt
      781 cggtaacctt atagcgctct cactaggcct gacaccaaat tttaagtcga acttcgactt
      841 agctgaagat gccaa
//
