LOCUS       sequence-67456-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For in vivo expression of Cas9 or generation of Cas9 mRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Gagnon JA, Valen E, Thyme SB, Huang P, Ahkmetova L, Pauli A, 
            Montague TG, Zimmerman S, Richter C, Schier AF
  TITLE     Efficient mutagenesis by Cas9 protein-mediated oligonucleotide 
            insertion and large-scale assessment of single-guide RNAs.
  JOURNAL   PLoS One. 2014 May 29;9(5):e98186. doi: 
            10.1371/journal.pone.0098186. eCollection 2014.
  PUBMED    24873830
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-05-29"; volume: "9(5):e98186. doi"; pages: " 
            10.1371/journal.pone.0098186. eCollection 2014"
COMMENT     Sequence Label: pCS2-Cas9
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ctctctctgg ggtgccctgc tgtctccacc gagctgagag aggtcgattc ttgtttcata
       61 gagccccgta attgactgat gaatcagtgt ggcgtccagg acctcctttg tagaggtgta
      121 ccgctttctg tctatggtgg tgtcgaagta cttgaaggct gcaggcgcgc ccaagttggt
      181 cagagtaaac aagtggataa tgttttctgc ctgctccctg atgggcttat ccctgtgctt
      241 attgtaagca gaaagcacct tatcgaggtt agcgtcggcg aggatcactc ttttggagaa
      301 ttcgcttatt tgctcgatga tctcatcaag gtagtgtttg tgttgttcca cgaacagctg
      361 cttctgctca ttatcttcgg gagacccttt gagcttttca tagtggctgg ccagatacaa
      421 gaaattaacg tatttagagg gcagtgccag ctcgttacct ttctgcagct cgcccgcact
      481 agcgagcatt cgtttccggc cgttttcaag ctcaaagaga gagtacttgg gaagcttaat
      541 gatgaggtct tttttgacct ctttatatcc tttcgcctcg agaaagtcga tggggttttt
      601 ttcgaagctt gatcgctcca tgattgtgat gcccagcagt tccttgacgc ttttgagttt
      661 tttagacttc cctttctcca ctttggccac aaccagtaca ctgtaagcga ctgtaggaga
      721 atcgaatccg ccgtatttct tggggtccca atcttttttg cgtgcgatca gcttgtcgct
      781 gttccttttc gggaggatac tttccttgga gaagcctccg gtctgtactt cggtcttttt
      841 aacgatgttc acctgcggca
//
