LOCUS       sequence-67474-e         606 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 606)
  AUTHORS   Huang L, Jin J, Deighan P, Kiner E, McReynolds L, Lieberman J
  TITLE     Efficient and specific gene knockdown by small interfering RNAs 
            produced in bacteria.
  JOURNAL   Nat Biotechnol. 2013 Apr;31(4):350-6. doi: 10.1038/nbt.2537. Epub 
            2013 Mar 10.
  PUBMED    23475073
REFERENCE   2  (bases 1 to 606)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.2537"; 
            journalName: "Nat Biotechnol"; date: "2013-04"; volume: "31"; issue:
            "4"; pages: "350-6"
COMMENT     Sequence Label: pGEX-4T-1-p19-T7-EGFPFL
FEATURES             Location/Qualifiers
     source          1..606
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             547..564
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     571..590
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        571..589
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctcccaatcg gactggttcc gcgtggatcc atggaacgag ctatacaagg aaacgacgct
       61 agggaacaag ctaacagtga acgttgggat ggaggatcag gaggtaccac ttctcccttc
      121 aaacttcctg acgaaagtcc gagttggact gagtggcggc tacataacga tgagacgaat
      181 tcgaatcaag ataatcccct tggtttcaag gaaagctggg gtttcgggaa agttgtattt
      241 aagagatatc tcagatacga caggacggaa gcttcactgc acagagtcct tggatcttgg
      301 acgggagatt cggttaacta tgcagcatct cgatttttcg gtttcgacca gatcggatgt
      361 acctatagta ttcggtttcg aggagttagt atcaccgttt ctggagggtc gcgaactctt
      421 cagcatctct gtgagatggc aattcggtct aagcaagaac tgctacagct tgccccaatc
      481 gaagtggaaa gtaatgtatc aagaggatgc cctgaaggta ctcaaacctt cgaaaaagaa
      541 agcgagcacc accaccacca ccactgaatc taatacgact cactataggg gagcttcatg
      601 gtgagc
//
