LOCUS       sequence-67707-e         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Friedman LJ, Gelles J
  TITLE     Mechanism of transcription initiation at an activator-dependent 
            promoter defined by single-molecule observation.
  JOURNAL   Cell. 2012 Feb 17;148(4):679-89. doi: 10.1016/j.cell.2012.01.018.
  PUBMED    22341441
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2012.01"; journalName: "Cell"; date: "2012-02-17- 
            17"; volume: "148"; issue: "4"; pages: "679-89"
COMMENT     Sequence Label: pSigma54-4
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             19..41
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             60..77
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             87..104
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 attccctcta gaaataattt tgtttaactt taagaaggag atataccatg ggcagcagcc
       61 atcatcatca tcatcacagc agctgcctgg ttccgcgcgg cagccatatg aagcaaggtt
      121 tgcaactcag gcttagccaa caactggcga tgacgccaca gctccaacag gcaattcgtc
      181 tgttgcagtt gtcgacgctg gaacttcagc aggagctaca gcaggcgctg gagagtaatc
      241 cgctgcttga gcaaatcgac actcatgaag aaatcgacac ccgcgaaacg caagacagtg
      301 aaacgctgga caccgccgac gcgctcgaac aaaaagagat gccggaagag ctgccgctcg
      361 atgccagttg ggacaccatt tacaccgctg gtacaccatc cggcaccagc ggtgactaca
      421 ttgacgacga gctgccggtc taccagggcg aaacgacgca gaccttgcag gattacctga
      481 tgtggcaggt tgagctgaca ccgttttccg acactgaccg cgctattgct acctctatcg
      541 tcgatgccgt tgatgaaacc ggttatctga ctgtcccgct ggaagatatt ctcgaaagta
      601 taggcgatga agagatcgac atcgacgaag ttgaagccgt ccttaagcgg atccaacggt
      661 ttgatccggt cggtgtggcg gcaaaagatc tgcgtgacgc tctgctgatc caactctccc
      721 aattcgataa gaccacgccg tggctggaag aggccagact gatcattagc gatcatctcg
      781 atctgttagc caatcacgac ttccgcactt taatgcgcgt cacgcgtctg aaagaagatg
      841 tgctgaaaga agccgtcaat ctgatccagt cgctcgatcc gcgccccggg cagtcgatcc
      901 agactggcga
//
