LOCUS       sequence-67708-e         892 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Hocine S, Raymond P, Zenklusen D, Chao JA, Singer RH
  TITLE     Single-molecule analysis of gene expression using two-color RNA 
            labeling in live yeast.
  JOURNAL   Nat Methods. 2013 Feb;10(2):119-21. doi: 10.1038/nmeth.2305. Epub 
            2012 Dec 23.
  PUBMED    23263691
REFERENCE   2  (bases 1 to 892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2305"; journalName: "Nat Methods"; date: "2013-02"; 
            volume: "10"; issue: "2"; pages: "119-21"
COMMENT     Sequence Label: pSH100 (YCplac33 MET25pro MCP-mCherry)
FEATURES             Location/Qualifiers
     source          1..892
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      400..409
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(551..569)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     819..838
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 ccccatccat actctagaat gctagccgtt aaaatggctt ctaactttac tcagttcgtt
       61 ctcgtcgaca atggcggaac tggcgacgtg actgtcgccc caagcaactt cgctaacggg
      121 atcgctgaat ggatcagctc taactcgcgt tcacaggctt acaaagtaac ctgtagcgtt
      181 cgtcagagct ctgcgcagaa tcgcaaatac accatcaaag tcgaggtgcc taaaggcgcc
      241 tggcgttcgt acttaaatat ggaactaacc attccaattt tcgccacgaa ttccgactgc
      301 gagcttattg ttaaggcaat gcaaggtctc ctaaaagatg gaaacccgat tccctcagca
      361 atcgcagcaa actccggcat ctacgcggat ccaccggtcg ccaccatggt gagcaagggc
      421 gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc
      481 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
      541 acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc
      601 ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc
      661 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
      721 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac
      781 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc
      841 atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct ga
//
