LOCUS       sequence-67721-e         759 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Hocine S, Raymond P, Zenklusen D, Chao JA, Singer RH
  TITLE     Single-molecule analysis of gene expression using two-color RNA 
            labeling in live yeast.
  JOURNAL   Nat Methods. 2013 Feb;10(2):119-21. doi: 10.1038/nmeth.2305. Epub 
            2012 Dec 23.
  PUBMED    23263691
REFERENCE   2  (bases 1 to 759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2305"; journalName: "Nat Methods"; date: "2013-02"; 
            volume: "10"; issue: "2"; pages: "119-21"
COMMENT     Sequence Label: pDZ513 (pHIS MET25pro PP7-PS-yeGFP)
FEATURES             Location/Qualifiers
     source          1..759
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(3..710)
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELY"
ORIGIN
        1 ttgtacaatt catccatacc atgggtaata ccagcagcag taacaaattc taacaagacc
       61 atgtggtctc tcttttcgtt tggatctttg gataaggcag attgagtgga taagtaatgg
      121 ttgtctggta acaagactgg accatcacca attggagtat tttgttgata atggtcagct
      181 aattgaacag aaccatcttc aatgttgtgt ctaattttga agttaacttt gataccattc
      241 ttttgtttgt cagccatgat gtaaacattg tgagagttat agttgtattc caatttgtga
      301 cctaaaatgt taccatcttc tttaaaatca atacctttta attcgattct attaactaag
      361 gtatcacctt caaacttgac ttcagctctg gtcttgtagt taccgtcatc tttgaaaaaa
      421 atagttcttt cttgaacata accttctggc atggcagact tgaaaaagtc atgttgtttc
      481 atatgatctg ggtatctcgc aaaacattga acaccataac cgaaagtagt gactaaggtt
      541 ggccatggaa ctggcaattt accagtagta caaataaatt ttaaggtcaa tttaccgtaa
      601 gtagcatcac cttcaccttc accggagaca gaaaatttgt gaccattaac atcaccatct
      661 aattcaacca aaattgggac aacaccagtg aataattctt cacctttaga cacgatatct
      721 ttgtacaatt catccatacc atgggtaata ccagcagca
//
