LOCUS       sequence-67816-e         781 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Fox JD, Routzahn KM, Bucher MH, Waugh DS
  TITLE     Maltodextrin-binding proteins from diverse bacteria and archaea are 
            potent solubility enhancers.
  JOURNAL   FEBS Lett. 2003 Feb 27. 537(1-3):53-7.
  PUBMED    12606030
REFERENCE   2  (bases 1 to 781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "FEBS Lett. 
            2003 Feb 27. 537(1-3):53-7."
COMMENT     Sequence Label: pKM1137
FEATURES             Location/Qualifiers
     source          1..781
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(87..93,94..111,112..117))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(142..266)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 aacggtggta tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct
       61 cgacggatcc taactcaaaa tccacacatt atacgagccg gaagcataaa gtgtaaagcc
      121 tggggtgcct aatgcggccg ccatagtgac tggatatgtt gtgttttaca gtattatgta
      181 gtctgttttt tatgcaaaat ctaatttaat atattgatat ttatatcatt ttacgtttct
      241 cgttcagctt ttttgtacaa acttgtgatg ggtttcgtca tctgcttttc agcatcagcc
      301 agagcggcat ccacggtttg acgaccatca acgatgttga tgatggcgtt tttcgcagag
      361 ctccaaaacg cgttcatttg tggaatgtta ggcataattt cgccgttcat ggcgttatcc
      421 atggtcgccg caatgcgcgc atccgcatcc agttcacgtt ggaaagagtt cagcgcaacc
      481 gcacctagtg gtttatcgtt gtttaccatg cgcagaccat cgttagtcag cagatagttt
      541 tcgatgaatt ccaccgccag atctttattt ggagaagcgg tgctgatgcc ggcggtgaga
      601 acgccaacga aaggttttga cgcttgaccg ttaaatttag gcaaagtggt cacaccgtag
      661 ttgatgcctg atttctcgat gttgccccaa gaccatggac cgttgatggt catcgcggta
      721 ttaccttggt taaaggcaga ctcagacact gagtaatcca tatctggaga gatcacgcct
      781 t
//
