LOCUS       sequence-67849-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Kiyomitsu T, Cheeseman IM
  TITLE     Cortical dynein and asymmetric membrane elongation coordinately 
            position the spindle in anaphase.
  JOURNAL   Cell. 2013 Jul 18;154(2):391-402. doi: 10.1016/j.cell.2013.06.010.
  PUBMED    23870127
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.06"; journalName: "Cell"; date: "2013-07-18- 
            18"; volume: "154"; issue: "2"; pages: "391-402"
COMMENT     Sequence Label: pTK81_GFP-4.1R
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             55..75
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             88..132
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 ctgtacaagt ccggagggga tcggtggagc tccaccggtg gcggccgctc tagagagaat
       61 ctttattttc agggcgccgc caaattcaaa gaaaccgctg ctgctaaatt cgaacgccag
      121 cacatggaca gcggaggtgg aggtactagt ctcgagatgc actgcaaggt ttctttgttg
      181 gatgacacag tttatgaatg tgttgtggag aaacatgcta agggacaaga tttgcttaaa
      241 cgagtatgtg agcatctcaa tcttttggaa gaagactatt ttggtctagc catttgggat
      301 aacgcaacct ctaagacatg gctggattcc gccaaagaaa taaaaaagca ggttcgtggt
      361 gtcccttgga attttacatt taatgtaaag ttttatccac ctgacccagc acagttaaca
      421 gaagacataa caagatatta tttatgtctt cagcttcggc aggacatagt tgcaggacgt
      481 ctgccctgtt cctttgcaac cttagcatta ttaggttctt acaccatcca gtctgaactg
      541 ggagactacg acccagaact ccatggcgtg gattatgtta gtgattttaa actggccccg
      601 aatcagacca aggaacttga agagaaggtc atggaactgc ataagtcata caggtccatg
      661 actccagctc aggctgactt ggagtttctt gagaatgcca aaaagttgtc tatgtatgga
      721 gttgatcttc ataaagcaaa ggacttggaa ggagtagata tcatcctagg tgtctgctct
      781 agtggccttc tggtttacaa agataagctg agaattaacc gcttcccttg gcccaaagtg
      841 ctgaagattt cttataaacg tagtagcttt
//
