LOCUS       sequence-67899-e         740 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Auyeung VC, Ulitsky I, McGeary SE, Bartel DP
  TITLE     Beyond Secondary Structure: Primary-Sequence Determinants License 
            Pri-miRNA Hairpins for Processing.
  JOURNAL   Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031.
  PUBMED    23415231
REFERENCE   2  (bases 1 to 740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-14- 
            14"; volume: "152"; issue: "4"; pages: "844-58"
COMMENT     Sequence Label: pcDNA3.2 mir-1-1 reporter cel-mir-50
FEATURES             Location/Qualifiers
     source          1..740
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    84..108
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(473..497)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agttaagcta tcaacaagtt tgtacaaaaa agcaggctca tatggcagat
      121 tgtgcaggag aacacggtga gctattttct ttattagaaa catttataaa ctaggactct
      181 cccaattcct cttcgattct atttttacta attctgaatt ccataattct atattctgta
      241 ttcctgcccg ccggccgctg atatgtctgg tattcttggg tttgaacttc cagcgttgaa
      301 cccgcatatt agacgtatcg acggccggcg gggcaggtaa tggctggtaa gtttggagag
      361 accgggggag tcccagagat gcatcgacca aaagccgaaa ttactgagct taatgaaaac
      421 tcttcacgtt ttcagcaaga aatcgtcgaa acagaattcg acgacaaagc ttacccagct
      481 ttcttgtaca aagtggttga tctagagggc ccgcagtggg gtcagcttct accggggcgg
      541 cgtcccgggg tcttggaact gcatgcagac tgcctgcttg ggaaacatac ttctttatat
      601 gcccatatgg acctgctaag ctatggaatg taaagaagta tgtatctcag gccgggacct
      661 ctctcgccgc actgaggggc actccacacc acgggggccg ccgcggccga cagccagcca
      721 ggcggctgtg gtgggggtga
//
