LOCUS       sequence-67904-e         704 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Auyeung VC, Ulitsky I, McGeary SE, Bartel DP
  TITLE     Beyond Secondary Structure: Primary-Sequence Determinants License 
            Pri-miRNA Hairpins for Processing.
  JOURNAL   Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031.
  PUBMED    23415231
REFERENCE   2  (bases 1 to 704)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-14- 
            14"; volume: "152"; issue: "4"; pages: "844-58"
COMMENT     Sequence Label: pcDNA3.2 mir-1-1 reporter dme-mir-279
FEATURES             Location/Qualifiers
     source          1..704
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    84..108
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(509..533)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agttaagcta tcaacaagtt tgtacaaaaa agcaggctag gaggaacaga
      121 aacgaactaa atgggtcgag cacattgtcg tgtattgtgt tttgcactat cgcttaataa
      181 ttgaaattaa agaggaggcg agggaaatgg agaacgcaaa aatcccatta taatggaatt
      241 gggactggag ctggaattgg aattcatact actgttttta gtgggtgggg gtccagtgtt
      301 tcacattgat tttcttagta tttgtgacta gatccacact cattaataac ggtagttcaa
      361 tcatcaagca cgtgttttct tgacaaactt cgcgtgtact tcattcgaaa agcaaaggga
      421 caaacaggtg gtgatctttg aaaaaaaaaa agagacgcaa ggtaattagt caatgtctcg
      481 cacgcacaca acgtcattca gacggaacac ccagctttct tgtacaaagt ggttgatcta
      541 gagggcccgc agtggggtca gcttctaccg gggcggcgtc ccggggtctt ggaactgcat
      601 gcagactgcc tgcttgggaa acatacttct ttatatgccc atatggacct gctaagctat
      661 ggaatgtaaa gaagtatgta tctcaggccg ggacctctct cgcc
//
