LOCUS       sequence-67933-e         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of T22N Rab8a in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Dupre DJ, Robitaille M, Ethier N, Villeneuve LR, Mamarbachi AM, 
            Hebert TE
  TITLE     Seven transmembrane receptor core signaling complexes are assembled 
            prior to plasma membrane trafficking.
  JOURNAL   J Biol Chem. 2006 Nov 10;281(45):34561-73. Epub 2006 Sep 7.
  PUBMED    16959776
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2006-11-10"; volume: "281(45)"; pages: "34561-73. 
            Epub 2006 Sep 7"
COMMENT     Sequence Label: Rab8-T22N(Flag) in pcDNA3.1neo
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     51..70
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        51..69
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             129..152
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(811..828)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 gcgagctctc tggctactag agaacccact gcttactggc ttatcgaaat taatacgact
       61 cactataggg agacccaagc tggctagcgt ttaaacttaa gcttggtacc gagctcggat
      121 ccaccatgga ctacaaggac gatgatgaca aggcgaagac ctacgattac ctgttcaagc
      181 tgctgctgat cggggactcg ggggtgggga agaactgtgt cctgttccgc ttctccgagg
      241 acgccttcaa ctccactttt atctccacca taggaattga ctttaaaatt aggaccatag
      301 agctcgatgg caagagaatt aaactgcaga tatgggacac agccggtcag gaacggtttc
      361 ggacgatcac aacggcctac tacaggggtg caatgggcat catgctggtc tacgacatca
      421 ccaacgagaa gtccttcgac aacatccgga actggattcg caacattgag gagcacgcct
      481 ctgcagacgt cgaaaagatg atactcggga acaagtgtga tgtgaatgac aagagacaag
      541 tttccaagga acggggagaa aagctggccc tcgactatgg aatcaagttc atggagacca
      601 gcgcgaaggc caacatcaat gtggaaaatg catttttcac tctcgccaga gatatcaaag
      661 caaaaatgga caaaaaattg gaaggcaaca gcccccaggg gagcaaccag ggagtcaaaa
      721 tcacaccgga ccagcagaag aggagcagct ttttccgatg tgttcttctg tgactcgagt
      781 ctagagggcc cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat
      841 ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc
      901 tttcctaata
//
