LOCUS       sequence-67943-e         894 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Giry-Laterriere M, Cherpin O, Kim YS, Jensen J, Salmon P
  TITLE     Polyswitch lentivectors: ""all-in-one"" lentiviral vectors for 
            drug-inducible gene expression, live selection, and recombination 
            cloning.
  JOURNAL   Hum Gene Ther. 2011 Oct;22(10):1255-67. Epub 2011 Sep 27.
  PUBMED    21761975
REFERENCE   2  (bases 1 to 894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Gene 
            Ther."; date: "2011-10"; volume: "22(10)"; pages: "1255-67. Epub 
            2011 Sep 27"
COMMENT     Sequence Label: pCLX-pTF-R1-DEST-R2-EBR65
FEATURES             Location/Qualifiers
     source          1..894
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             97..492
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             502..561
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
ORIGIN
        1 ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg
       61 tttgccgcca gaacacaggt gtcgtgacgc ggatccatgg ccaagccttt gtctcaagaa
      121 gaatccaccc tcattgaaag agcaacggct acaatcaaca gcatccccat ctctgaagac
      181 tacagcgtcg ccagcgcagc tctctctagc gacggccgca tcttcactgg tgtcaatgta
      241 tatcatttta ctgggggacc ttgtgcagaa ctcgtggtgc tgggcactgc tgctgctgcg
      301 gcagctggca acctgacttg tatcgtcgcg atcggaaatg agaacagggg catcttgagc
      361 ccctgcggac ggtgccgaca ggtgcttctc gatctgcatc ctgggatcaa agccatagtg
      421 aaggacagtg atggacagcc gacggcagtt gggattcgtg aattgctgcc ctctggttat
      481 gtgtgggagg gcggatcagg tcaatgtact aactacgctt tgttgaagct tgccggcgat
      541 gttgaaagta accccggtcc tatgtccaga ctggacaaga gcaaagtcat aaacggcgct
      601 ctggaattac tcaatggagt cggtatcgaa ggcctgacga caaggaaact cgctcaaaag
      661 ctgggagttg agcagcctac cctgtactgg cacgtgaaga acaagcgggc cctgctcgat
      721 gccctgccaa tcgagatgct ggacaggcat catacccact tctgccccct ggaaggcgag
      781 tcatggcaag actttctgcg gaacaacgcc aagtcattcc gctgtgctct cctctcacat
      841 cgcgacgggg ctaaagtgca tctcggcacc cgcccaacag agaaacagta cgaa
//
