LOCUS       sequence-67945-e         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of N124I Rab1a in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Dupre DJ, Robitaille M, Ethier N, Villeneuve LR, Mamarbachi AM, 
            Hebert TE
  TITLE     Seven transmembrane receptor core signaling complexes are assembled 
            prior to plasma membrane trafficking.
  JOURNAL   J Biol Chem. 2006 Nov 10;281(45):34561-73. Epub 2006 Sep 7.
  PUBMED    16959776
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2006-11-10"; volume: "281(45)"; pages: "34561-73. 
            Epub 2006 Sep 7"
COMMENT     Sequence Label: pcDNA3 Flag Rab1a N124I
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     51..70
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        51..69
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             155..178
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gcagagctct ctggctactg agaacccact gcttactggc ttatcgaaat taatacgact
       61 cactataggg agacccaagc ttggtaccga gctcggatcc actagtaacg gccgccagtg
      121 tgctggaatt cggcttgctg tcgacaccat gggggactac aaggacgacg atgacaaggg
      181 gggtagcggt ggatccagca tgaatcccga atatgattat ttattcaagt tacttctgat
      241 tggcgattct ggggttggaa agtcctgcct tctccttagg tttgcagatg atacgtatac
      301 ggaaagctac atcagcacaa ttggtgtgga tttcaagata cgaactatag agttagatgg
      361 gaaaacaatc aagctacaga tatgggacac agcaggccag gaaagatttc gaacaatcac
      421 ttccagttat tacagaggag cccatggcat catagttgtg tatgatgtga cagatcagga
      481 gtccttcaat aacgttaaac agtggctgca ggagatagat cgctacgcca gtgaaaatgt
      541 caacaagttg ttggtaggga tcaaatgtga cctgaccaca aagaaagtag tagactacac
      601 aacagcaaag gaatttgcag attcccttgg aattccattt ttggaaacca gtgctaagaa
      661 cgcaacgaat gtagaacagt ctttcatgac gatggcagct gagattaaaa agcgaatggg
      721 tcctggagct acagctggtg gtgccgagaa gtccaatgtt aaaatccaga gcactccagt
      781 caagcagtca ggtggaggct gctgctaaaa tccgcctccg tccttttctc acagcaatga
      841 at
//
