LOCUS       sequence-67991-e         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Machida K, Thompson CM, Dierck K, Jablonowski K, Karkkainen S, Liu 
            B, Zhang H, Nash PD, Newman DK, Nollau P, Pawson T, Renkema GH, 
            Saksela K, Schiller MR, Shin DG, Mayer BJ
  TITLE     High-throughput phosphotyrosine profiling using SH2 domains.
  JOURNAL   Mol Cell. 2007 Jun 22;26(6):899-915.
  PUBMED    17588523
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell.";
            date: "2007-06-22"; volume: "26(6)"; pages: "899-915"
COMMENT     Sequence Label: pGEX p85b(C)-SH2
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             5..28
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(496..518)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(663..681)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        749..853
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggatctggaa gttctgttcc aggggcccct gggatccggg cgcgccatgg acgaggacga
       61 tctcccgcac cacgaggaac gcacttggta cgtgggcaag atcaaccgca cgcaggcaga
      121 ggagatgctg agtggcaagc gggatggcac cttcctcatc cgcgagagca gccagcgggg
      181 ctgctacgcc tgctccgtgg tagtggacgg cgacaccaag cactgcgtca tctaccgcac
      241 ggccaccggc ttcggcttcg cggagcccta caacctgtac gggtcgctga aggagctggt
      301 gctgcactac cagcacgcct cgctggtgca gcacaacgac gcgctcaccg tcaccctggc
      361 gcacccagtg cgcgccccgg gccccggccc gccgcctgcc gcccgcttaa ttaacgaatt
      421 cccgggtcga ctcgagcggc cgcatcgtga ctgactgacg atctgcctcg cgcgtttcgg
      481 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
      541 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
      601 gggcgcagcc atgacccagt cacgtagcga tagcggagtg tataattctt gaagacgaaa
      661 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
      721 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
      781 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
      841 aaaaaggaag agtatgagta ttca
//
