LOCUS       sequence-68002-e         916 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Crocker J, Stern DL
  TITLE     TALE-mediated modulation of transcriptional enhancers in vivo.
  JOURNAL   Nat Methods. 2013 Aug;10(8):762-7. doi: 10.1038/nmeth.2543. Epub 
            2013 Jun 30.
  PUBMED    23817068
REFERENCE   2  (bases 1 to 916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2543"; journalName: "Nat Methods"; date: "2013-08"; 
            volume: "10"; issue: "8"; pages: "762-7"
COMMENT     Sequence Label: pJC-TALE-VP64
FEATURES             Location/Qualifiers
     source          1..916
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     22..44
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     36..53
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     37..53
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     63..82
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        63..81
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(129..149)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(129..147)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(168..184)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(168..184)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(181..203)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    192..208
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(216..222,223..240,241..246))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    261..282
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg
       61 cgtaatacga ctcactatag ggcgaattgg gtaccgggcc ccccctcgag gtcctccagc
      121 ttttgttccc tttagtgagg gttaattgcg cgcttggcgt aatcatggtc atagctgttt
      181 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
      241 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
      301 cccgctttcc accggtcgtc tccaacgacc acctcgtcgc cttggcctgc ctcggcggac
      361 gtcctgccat ggatgcagtg aaaaagggat tgccgcacgc gccggaattg atcagaagag
      421 tcaatcgccg tattggcgaa cgcacgtccc atcgcgttgc cgactacgcg caagtggttc
      481 gcgtgctgga gtttttccag tgccactccc acccagcgta cgcatttgat gaggccatga
      541 cgcagttcgg gatgagcagg aacgggttgg tacagctctt tcgcagagtg ggcgtcaccg
      601 aactcgaagc ccgcggtgga acgctccccc cagcctcgca gcgttgggac cgtatcctcc
      661 aggcatcagg gatgaaaagg gccaaaccgt cccctacttc agctcaaaca ccggatcagg
      721 cgtctttgca tgcattcgcc gattcgctgg agcgtgacct tgatgcgccc agcccaatgc
      781 acgagggaga tcagacgcgg gcaagcagcc gtaaacggtc ccgatcggat cgtgctgtca
      841 ccggcccctc cgcacagcag gctgtcgagg tgcgcgttcc cgaacagcgc gatgcgctgc
      901 atttgcccct cagctg
//
