LOCUS       sequence-68033-e         901 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 901)
  AUTHORS   DiCostanzo AC, Thompson JR, Peterson FC, Volkman BF, 
            Ramirez-Alvarado M
  TITLE     Tyrosine residues mediate fibril formation in a dynamic light chain 
            dimer interface.
  JOURNAL   J Biol Chem. 2012 Aug 10;287(33):27997-8006. doi: 
            10.1074/jbc.M112.362921. Epub 2012 Jun 27.
  PUBMED    22740699
REFERENCE   2  (bases 1 to 901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.362921"; journalName: "J Biol Chem"; date: 
            "2012-08-10- 10"; volume: "287"; issue: "33"; pages: "27997-8006"
COMMENT     Sequence Label: pET12a-AL-103 Y32F Y96F
FEATURES             Location/Qualifiers
     source          1..901
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     59..78
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        59..77
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             109..131
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(585..603)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      599..646
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 cggtgagccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaatta
       61 atacgactca ctatagggag accacaacgg tttccctcta gaaataattt tgtttaactt
      121 taagaaggag atatacatat gcgggcgaaa ctcctaggaa tagtcctgac aacccctatc
      181 gcgatcagct cttttgcgtc gacggacatc cagatgaccc agtctccatc ctccctgtct
      241 gcatctgtag gagacagagt caccatcact tgccaggcga gtcaggacat tagtaatttt
      301 ttaatttggt atcaacagaa accagggaaa gcccctaaac tcctgatcta cgatgcatcc
      361 aatttggaaa caggggtccc atcaaggttc agtggaagtg gatctgggac agattttact
      421 ttcaccatca gcagcctgca gcctgaagat attgcaacat attactgtca acagtatcat
      481 aatctccctc cgttcacttt tggcccgggg accaagctgg agatcaaacg ataaggatcc
      541 ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact
      601 agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac
      661 tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc
      721 caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa
      781 cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg
      841 cgatgctgtc ggaatggacg atatcccgca agaggccggc agtacggcta ccagctatgc
      901 c
//
