LOCUS       sequence-68069-e         618 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 618)
  TITLE     miR-224
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 618)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pTWEEN-miR-224
FEATURES             Location/Qualifiers
     source          1..618
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(44..554)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(107..125)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
ORIGIN
        1 aagctctcgc ccttgctacc atggtggcga ccggtggatc cccctgggga gagaggtcgg
       61 tgattcggtc aacgagggag ccgactgccg acgtgcgctc cggaggcttg cagaatgcgg
      121 aacaccgcgc gggcaggaac agggcccaca ctaccgcccc acaccccgcc tcccgcaccg
      181 ccccttcccg gccgctgctc tcggcgcgcc ctgctgagca gccgctattg gccacagccc
      241 atcgcggtcg gcgcgctgcc attgctccct ggcgctgtcc gtctgcgagg gtactagtga
      301 gacgtgcggc ttccgtttgt cacgtccggc acgccgcgaa ccgcaaggaa ccttcccgac
      361 ttaggggcgg agcaggaagc gtcgccgggg ggcccacaag ggtagcggcg aagatccggg
      421 tgacgctgcg aacggacgtg aagaatgtgc gagacccagg gtcggcgccg ctgcgtttcc
      481 cggaaccacg cccagagcag ccgcgtccct gcgcaaaccc agggctgcct tggaaaaggc
      541 gcaaccccaa ccccgtggaa ttcgatatca agcttctcga gttctgaacc cttaatcgcc
      601 gagcacgtaa gtatgctc
//
