LOCUS       sequence-68080-e         909 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses mammalian codon optimized Cas9 nuclease with C-term 3X 
            FLAG from CMV and T7 promoters.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Joung JK
  TITLE     Joung lab unpublished CRISPR plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: JDS246
FEATURES             Location/Qualifiers
     source          1..909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(19..84)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(91..111)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gaccggtcat cctgcagcct tgtcatcgtc atccttgtaa tcgatgtcat gatctttata
       61 atcaccgtca tggtctttgt agtcgctcga gactttcctc ttcttcttgg gggatccgtc
      121 acccccaagc tgtgacaaat ctatccgagt ttcatataat cccgtgatgg attggtgaat
      181 cagtgtcgcg tctagcacct ccttggtaga agtgtatcgt ttgcgatcta tcgttgtgtc
      241 aaaatacttg aatgcggctg gagcgccgag gttggtaaga gtaaacaaat ggataatatt
      301 ttccgcctgc tcacgtatgg gtttatccct gtgcttgttg tatgcgctta atactttgtc
      361 cagattggca tcagctagga tgactctctt actgaattcc gaaatttgct ctatgatttc
      421 gtcgagataa tgtttgtgct gctcaacaaa aagttgcttc tgttcgttat cttcaggtga
      481 acctttcaac ttctcgtaat gggacgctaa atacaggaaa ttcacgtatt tagacggtag
      541 tgcgagttcg ttcccctttt gaagctctcc ggcgctagcc aacatccgtt ttcggccatt
      601 ttctaactca aacagactat actttggtag tttaattatg agatcctttt ttacttcctt
      661 gtaacctttc gcctcaagga agtcgatggg gttcttttca aaagacgagc gctccataat
      721 cgttatcccc aataattctt tgactgactt cagtttcttg gattttccct tctcaacttt
      781 tgccactact aggacagaat aggcaactgt agggctatcg aagccaccgt actttttcgg
      841 gtcccagtcc tttttacgag cgatgagcta atcactattc cnttttggaa gaatcgattc
      901 ctttgaaaa
//
