LOCUS       sequence-68215-e         942 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Carmine intensiometric genetically encoded Ca2+-indicators for 
            optical imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Wu J, Liu L, Matsuda T, Zhao Y, Rebane A, Drobizhev M, Chang YF, 
            Araki S, Arai Y, March K, Hughes TE, Sagou K, Miyata T, Nagai T, Li 
            WH, Campbell RE
  TITLE     Improved Orange and Red Ca Indicators and Photophysical 
            Considerations for Optogenetic Applications.
  JOURNAL   ACS Chem Neurosci. 2013 Mar 19.
  PUBMED    23452507
REFERENCE   2  (bases 1 to 942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Neurosci. 2013 Mar 19."
COMMENT     Sequence Label: CMV-mito-CAR-GECO1
FEATURES             Location/Qualifiers
     source          1..942
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..90
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             112..186
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             235..291
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKAGHAVRAIGRLSS"
ORIGIN
        1 cctctagact cgagcatgtc tgttctgact cctctgctgc tccggggtct cacaggttcc
       61 gcaagaagac tccccgtgcc tagggccaaa attcattcac tgggggaccc catgagcgtg
      121 ctcaccccac tcctgctgcg ggggctgacc ggcagcgcta ggcggctgcc agtccccagg
      181 gccaagatcc acagtctcgg cgatcccaag gatccaacca tggtcgactc ttcacgtcgt
      241 aagtggaata aggcaggtca cgcagttaga gctataggtc ggctgagctc acccgtggtt
      301 tccgagcgga tgtaccccga ggacggcgcc ctgaagagcg agatcaagaa ggggctgagg
      361 ctgaaggacg gcggccacta cgccgccgag gtcaagacca cctacaaggc caagaagccc
      421 gtgcagctgc ccggcgccta catcgtcgac atcaagttgg acatcgtgtc ccacaacgag
      481 gactacacca tcgtggaaca gtgcgaacgc gccgagggcc gccactccac cggcggcatg
      541 gacgagctgt acaagggagg tacaggcggg agtctggtga gcaagggcgt ggaggataac
      601 atggccatcg tcaaggagtt catgcgcttc aagacgcaca tagagggctc cgtgaacggc
      661 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgaggcctt tcagaccgct
      721 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag
      781 attatgtacg gctccaaggc ctacattaag cacccagccg acatccccga ctacttcaag
      841 ctgtccttcc ccgagggctt caggtgggag cgcgtgatga acttcgagga cggcggcatt
      901 attcacgtta accaggactc ctccctgcag gacggcgtat tc
//
