LOCUS       sequence-68253-e         903 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Ruas JL, White JP, Rao RR, Kleiner S, Brannan KT, Harrison BC, 
            Greene NP, Wu J, Estall JL, Irving BA, Lanza IR, Rasbach KA, Okutsu 
            M, Nair KS, Yan Z, Leinwand LA, Spiegelman BM
  TITLE     A PGC-1alpha isoform induced by resistance training regulates 
            skeletal muscle hypertrophy.
  JOURNAL   Cell. 2012 Dec 7;151(6):1319-31. doi: 10.1016/j.cell.2012.10.050.
  PUBMED    23217713
REFERENCE   2  (bases 1 to 903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2012.10"; journalName: "Cell"; date: "2012-12-7- 7";
            volume: "151"; issue: "6"; pages: "1319-31"
COMMENT     Sequence Label: pcDNA3.1-Flag-PGC-1alpha1
FEATURES             Location/Qualifiers
     source          1..903
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             106..129
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct tggtaccgag ctcggatcca ccatggacta caaagacgat
      121 gacgacaagg cggccgcgat ggcttgggac atgtgcagcc aagactctgt atggagtgac
      181 atagagtgtg ctgctctggt tggtgaggac cagcctcttt gcccagatct tcctgaactt
      241 gacctttctg aacttgatgt gaatgacttg gatacagaca gctttctggg tggattgaag
      301 tggtgtagcg accaatcgga aatcatatcc aaccagtaca acaatgagcc tgcgaacata
      361 tttgagaaga tagatgaaga gaatgaggca aacttgctag cggtcctcac agagacactg
      421 gacagtctcc ccgtggatga agacggattg ccctcatttg atgcactgac agatggagcc
      481 gtgaccactg acaacgaggc cagtccttcc tccatgcctg acggcacccc tccccctcag
      541 gaggcagaag agccgtctct acttaagaag ctcttactgg caccagccaa cactcagctc
      601 agctacaatg aatgcagcgg tcttagcact cagaaccatg cagcaaacca cacccacagg
      661 atcagaacaa accctgccat tgttaagacc gagaattcat ggagcaataa agcgaagagc
      721 atttgtcaac agcaaaagcc acaaagacgt ccctgctcag agcttctcaa gtatctgacc
      781 acaaacgatg accctcctca caccaaaccc acagaaaaca ggaacagcag cagagacaaa
      841 tgtgcttcca aaaagaagtc ccatacacaa ccgcagtcgc aacatgctca agccaaacca
      901 aca
//
