LOCUS       sequence-68276-e         945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To express Fun30 in trans in budding yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Neves-Costa A, Will WR, Vetter AT, Miller JR, Varga-Weisz P
  TITLE     The SNF2-family member Fun30 promotes gene silencing in 
            heterochromatic loci.
  JOURNAL   PLoS One. 2009 Dec 1;4(12):e8111. doi: 10.1371/journal.pone.0008111.
  PUBMED    19956593
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-12-1"; volume: "4(12):e8111. doi"; pages: " 
            10.1371/journal.pone.0008111"
COMMENT     Sequence Label: pFA1
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     24..43
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        24..42
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    95..106
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
ORIGIN
        1 ccggatcgga ctactagcag ctgtaatacg actcactata gggaatatta agctcgccct
       61 tgtcgacgaa aacatgagtg gttcgcattc aaatgatgag gatgacgtag tgcaagtgcc
      121 cgagacgtcc tctcccacca aggtagcatc gtcgtctccc ttaaagccta cttcgccaac
      181 agttccggat gcaagtgtgg cgtctttgag aagcaggttt actttcaagc cttcagatcc
      241 cagcgaagga gctcatactt cgaagccgct cccatctggg agtcctgagg tagcactggt
      301 taaccttgcg agagagttcc ccgatttctc tcaaactctg gtgcaggctg ttttcaaatc
      361 taactctttt aacttacagt ctgccaggga acgtcttaca agattgaggc agcaaagaca
      421 aaattggaca tggaacaaga acgcatctcc caagaagtca gaaaccccgc cacctgtcaa
      481 gaagtcatta ccactggcaa acacaggccg tttatcatct atccatggca atatcaacaa
      541 caaatcctcc aagattaccg tggccaaaca gaaaacgtcc atttttgacc gttactcaaa
      601 cgtcatcaac cagaaacaat acacttttga gctgccaact aacttgaata tagactcgga
      661 ggcactgagc aagttgcccg tgaactacaa caaaaagaga aggctggtaa gggcagatca
      721 gcatccaatt ggcaagtctt atgagtcatc cgctacacaa ttaggttctg caagagagaa
      781 actactggcg aaccgcaaat acggtcgtca tgcaaacgac aacgatgaag aggaggaaga
      841 gagtatgatg acggacgatg acgatgcaag tggcgacgac tacacagaat ccacgccgca
      901 gataaatctg gatgaacaag ttttacagtt tattaatgac tctga
//
