LOCUS       sequence-68544-e         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Human pSico-based U6 vector containing murine U6 promoter and sgRNA 
            targeting GFP (NT1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Gilbert LA, Larson MH, Morsut L, Liu Z, Brar GA, Torres SE, 
            Stern-Ginossar N, Brandman O, Whitehead EH, Doudna JA, Lim WA, 
            Weissman JS, Qi LS
  TITLE     CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in 
            Eukaryotes.
  JOURNAL   Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 
            10.1016/j.cell.2013.06.044.
  PUBMED    23849981
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2013 
            Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044."
COMMENT     Sequence Label: pU6-sgGFP-NT1
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(291..324)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     328..348
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     misc_RNA        431..506
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
       61 gagatccagt ttggttagta ccgggcccgc tctagagatc cgacgcgcca tctctaggcc
      121 cgcgccggcc ccctcgcacg gacttgtggg agaagctcgg ctactcccct gccccggtta
      181 atttgcatat aatatttcct agtaactata gaggcttaat gtgcgataaa agacagataa
      241 tctgttcttt ttaatactag ctacatttta catgataggc ttggatttct ataacttcgt
      301 atagcataca ttatacgaag ttataaacag cacaaaagga aactcaccct aactgtaaag
      361 taattgtgtg ttttgagact ataagtatcc cttggagaac caccttgttg gaatagctca
      421 gaggccgagg gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac
      481 ttgaaaaagt ggcaccgagt cggtgctttt tttctcgagt actaggatcc attaggcggc
      541 cgcgtggata accgtattac cgccatgcat tagttattaa tagtaatcaa ttacggggtc
      601 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
      661 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
      721 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
      781 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
      841 taaatggccc gcc
//
