LOCUS       sequence-68601-e         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein overexpression with the modified LacIWF repressor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Gatti-Lafranconi P, Dijkman WP, Devenish SR, Hollfelder F
  TITLE     A single mutation in the core domain of the lac repressor reduces 
            leakiness.
  JOURNAL   Microb Cell Fact. 2013 Jul 8;12:67. doi: 10.1186/1475-2859-12-67.
  PUBMED    23834731
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2013-07-8"; volume: "12:67. doi"; pages: " 
            10.1186/1475-2859-12-67"
COMMENT     Sequence Label: pLIGFP_W220F
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc
       61 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
      121 gtttgcgtat tgggtgctag cgtggttttt cttttcacca gtgagacggg caacagctga
      181 ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc
      241 agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg
      301 gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga ctcggtaatg
      361 gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg
      421 ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactgaa gtcgccttcc
      481 cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc
      541 agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg gtgacccaat
      601 gcgaccagat gctccacgcc cagtcgcgta ccgtcttcat gggagaaaat aatactgttg
      661 atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca ggcagcttcc
      721 acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact gacgcgttgc
      781 gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc taccatcgac
      841 accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac aatttgcgac
      901 ggcgcgtgca gggccagact
//
