LOCUS       sequence-68771-e         799 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial targeting.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Alexeyev MF
  TITLE     The pKNOCK series of broad-host-range mobilizable suicide vectors 
            for gene knockout and targeted DNA insertion into the chromosome of 
            gram-negative bacteria.
  JOURNAL   Biotechniques. 1999 May;26(5):824-6, 828.
  PUBMED    10337469
REFERENCE   2  (bases 1 to 799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biotechniques."; date: "1999-05"; volume: "26(5)"; pages: "824-6, 
            828"
COMMENT     Sequence Label: pKNOCK-Tc
FEATURES             Location/Qualifiers
     source          1..799
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     73..89
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     73..89
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(123..139)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(124..140)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 aagcaatttt gagtgacaca ggaacactta acggctgaca tgggaattcc cctccaccgc
       61 ggtggcggcc gctctagaac tagtggatcc cccgggctgc aggaattcga tatcaagctt
      121 atcgataccg tcgacctcga gggggggccc ggtaccgagg acgcgtcagc ttgatcaagg
      181 gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt
      241 gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca
      301 ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac
      361 ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccaatgatc
      421 gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct
      481 acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga
      541 atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc
      601 gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga
      661 ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg
      721 atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc
      781 acctgtccta cgagttgca
//
