LOCUS       sequence-68837-e         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible lentiviral gene silencing vector. Insert PheSGly294, (XhoI
            to EcoR1; 1.4kb), can be replaced w mir30 based hairpin of interest.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Meerbrey KL, Hu G, Kessler JD, Roarty K, Li MZ, Fang JE, 
            Herschkowitz JI, Burrows AE, Ciccia A, Sun T, Schmitt EM, Bernardi 
            RJ, Fu X, Bland CS, Cooper TA, Schiff R, Rosen JM, Westbrook TF, 
            Elledge SJ
  TITLE     The pINDUCER lentiviral toolkit for inducible RNA interference in 
            vitro and in vivo.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Mar 1;108(9):3665-70. doi: 
            10.1073/pnas.1019736108. Epub 2011 Feb 9.
  PUBMED    21307310
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1019736108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-03-1- 1"; volume: "108"; issue: "9"; pages: "3665-70"
COMMENT     Sequence Label: pINDUCER11 (miR-RUG)
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    127..160
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ttttgtgaag ttttttaggc accttttgaa atgtaatcat ttgggtcaat atgtaatttt
       61 cagtgttaga ctagtaaatt gtccgctaaa ttctggccgt ttttggcttt tttgttagac
      121 ggatccataa cttcgtataa tgtatgctat acgaagttat gccaccatgt ctagactgga
      181 caagagcaaa gtcataaacg gagctctgga attactcaat ggtgtcggta tcgaaggcct
      241 gacgacaagg aaactcgctc aaaagctggg agttgagcag cctaccctgt actggcacgt
      301 gaagaacaag cgggccctgc tcgatgccct gccaatcgag atgctggaca ggcatcatac
      361 ccacttctgc cccctggaag gcgagtcatg gcaagacttt ctgcggaaca acgccaagtc
      421 ataccgctgt gctctcctct cacatcgcga cggggctaaa gtgcatctcg gcacccgccc
      481 aacagagaaa cagtacgaaa ccctggaaaa tcagctcgcg ttcctgtgtc agcaaggctt
      541 ctccctggag aacgcactgt acgctctgtc cgccgtgggc cactttacac tgggctgcgt
      601 attggaggaa caggagcatc aagtagcaaa agaggaaaga gagacaccta ccaccgattc
      661 tatgccccca cttctgagac aagcaattga gctgttcgac cggcagggag ccgaacctgc
      721 cttccttttc ggcctggaac taatcatatg tggcctggag aaacagctaa agtgcgaaag
      781 cggcgggccg accgacgccc ttgacgattt tgacttagac atgctcccag ccgatgccct
      841 tgacgacttt gaccttgata tgctgcctgc tgac
//
