LOCUS       sequence-68879-e        9438 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length RON6 with no 
            N-linked glycans upon cotransfection with BirA in mammalian cells. 
            Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9438)
  AUTHORS   Crosnier C, Wanaguru M, McDade B, Osier FH, Marsh K, Rayner JC, 
            Wright GJ
  TITLE     A library of functional recombinant cell surface and secreted 
            Plasmodium falciparum merozoite proteins.
  JOURNAL   Mol Cell Proteomics. 2013 Sep 16.
  PUBMED    24043421
REFERENCE   2  (bases 1 to 9438)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics. 2013 Sep 16."
COMMENT     Sequence Label: RON6-bio
FEATURES             Location/Qualifiers
     source          1..9438
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        154..533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        534..737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     688..708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     734..758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1239..1258
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1281..1290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    4818..4873
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4872..4891)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(7109..7127)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7195..7299
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7300..7368,7369..8160)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7518..7537)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8331..8919
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8820..8839
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9073..9090
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9207..9228
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9243..9248,9249..9266,9267..9273)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9281..9297
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9286..9308
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9305..9321
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9305..9321
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat
       61 aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt
      121 atattggctc atgtccaata tgaccgccat gttgacattg attattgact agttattaat
      181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc
      421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac
      481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      541 ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      661 aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg
      721 tctatataag cagagctcgt ttagtgaacc gtcagatcct cactctcttc cgcatcgctg
      781 tctgcgaggg ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt
      841 actcttggat cggaaacccg tcggcctccg aacggtactc cgccaccgag ggacctgagc
      901 gagtccgcat cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg
      961 caaggtaggc tgagcaccgt ggcgggcggc agcgggtggc ggtcggggtt gtttctggcg
     1021 gaggtgctgc tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgaggtg
     1081 aggtgtggca ggcttgagat ccagctgttg gggtgagtac tccctctcaa aagcgggcat
     1141 tacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc
     1201 cgatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt
     1261 ccactcccag gtccaagttt gccaccatgg agtttcagac ccaggtactc atgtccctgc
     1321 tgctctgcat gtctggtgcg gccgctttcc tgcggaacaa agagcacgcc aacctgatca
     1381 acagctacaa cgacatcgtc gaggacatca acatcaagaa agaggaaaag agcagcagcg
     1441 agcccccatt catccccatc aagaacaaga tcgacaacgt gcacaccaag aacaacaacc
     1501 agtacaacct gcacaacaac aaggccaaca aagcccacct gacctacggc acccacacca
     1561 gctttctgca gaactgcgcc atcaacgact gcgtggacgt ggacaacaag gacagcgaga
     1621 tcaacaatat cgccaaagag aaggacgaca acaacaacaa caatggcgcc aagcagatcg
     1681 aggaaaagaa caaaatcaac aaggccgacc tgcaccggca gaacgagctg aacctgcaga
     1741 gcggcaagaa cgagcaggat atcaacaaga acgagaaggg caagcaggac atcagcaaca
     1801 gcaacgccga gaacaagaag gacgtcaaag agggcgtcaa agagctggaa gagaagaaga
     1861 aagaagagaa gatcagcgac gaccacaaag tggaagagaa taaaaagtcc gacgatcaca
     1921 aagtcgagga aaacaagaag tctgacgacc ataaggtcga agaaaacaag aaaagcgacg
     1981 atcacaagat cgaagaggtc aaaaaggtcg aggaacacga ggaagatgag gaagaggaca
     2041 agaaagagaa gaaatctgaa aacaaaaaca aggatgagaa caaggacgag aatgacgagg
     2101 acaacgacga gatctccgac gaggacgagg tggacgacga cgtggaagaa gataagaacg
     2161 aaaacgacga catcgacgac gataagaaag agacagacaa gacccacctg gaagaggaag
     2221 agaacgagat catcgagaaa gagttcagcg acaagaagaa gaacggcaag aacaaggata
     2281 ccaagaaaga aaagagcaag gacaccgaaa aagagaagtc caaggacatt gagaaagaga
     2341 aaagcaaaga caaagagaaa gaaaaatcta aggacaaaga aaaagagaag gggaaggaca
     2401 aagagaaaga gaaatccaaa gacatcgaaa aagaaaaaga aaaggacaag gatatcgaga
     2461 aagagaagtc taaagatacc gccaaagaaa aagaaaaaga caaagacatt gaaaaagaaa
     2521 agtccaagga tatggaaaag ctgaagaaca agcagaacga cgagaagaaa aaggacgata
     2581 acgagaaaaa gaagaatgat aagcaggata ttcacgacga caacgatgat gagaacgaca
     2641 tggaagagat tgaggaaaat gacgacgaag aggacgagga tgaggacatg gaaaacaaaa
     2701 agaagaaaaa aaagggcaaa aacgggaatg agaacggaaa cgagaatggc gccgagaacg
     2761 gcaatgagaa cggcaatgaa aacgggaatg agaatgagaa caagaatgag gccgagaatg
     2821 agaacgaaaa tgagaacgaa aacgagaatg ggaacgaaaa cgaaaatgag aaagagaatg
     2881 agaaggacaa gaacatcaaa gaaatcgaga acgtggccaa cgccaacaaa gagaactacg
     2941 agaagatcaa caagaattcc gagatcacca tcaccaagag caacatcgac atctataaca
     3001 acaaccggaa caacgatatc gacaaagtga acaaccacat cttcaccaac cagcagaaga
     3061 agcacaacct ccacaacgag cagaacaagt tcaacgaggc cctgaatgtg gccaccaacc
     3121 acaagaacca ctacgaggaa aaaaagaaat acgagtccaa catgttcaac gtggacaagc
     3181 ggatgcacaa gaacctggcc agcatggaca ccatcctgca caacctgaac gacaagctga
     3241 gccaccacaa ggacctgaag aacagagagc tgaagctgaa gttcgaggcc atgagccgga
     3301 tcaaagagta caagatctat aacgagctga tccagaagtc cgtcgagatc ctgaccctgc
     3361 ggctgaccaa gatcaacgac gagctgaaga agctgaagga catctccgag aacgccctgc
     3421 agaagtacat caacgagaaa ggctacggcc tgatgaagca ctacaacttc cccgagtaca
     3481 acaagctcga ggaagaaaag atcatgaagc ggagcaagat cacctggaat gagaaaaaga
     3541 gccaccacct gtactgcccc atggaatgca acaaagagcg gtgctacaac cggcccagag
     3601 gccctaccca gtgctacaag ctggaacaga tcggccggaa catcgagaca atctgcgagc
     3661 ccttcatcaa cgagcacaac ggcgcctgca gacccgactt ccaccactgc gccattgccg
     3721 agcccgagcg gaacaagaag tacagcatct acgccaccga cgacgtgcac ttcgtgccca
     3781 gccagttcgt gaccatcaag ggctacaatc tgcacgagtg cctgcagttt ctggtggtca
     3841 agaagaacag cgcctgcggc cccatgacca tcgagaagaa cctgatggaa agcgaggaaa
     3901 tcctgaaaga acccgtgctg agcaaggtgc tgtacaacga gatcctgttc gagaacatca
     3961 agatcagcac ccctggcgag tacaatatct gcctggccca gttctaccag gaccccgaga
     4021 aggatgacat cctgagcgag aactccgcca acaagtataa gaagaaaaag aaaaacgata
     4081 tgaaggtgct gggcatcgac accatcggca ccctgtacgt gctgcccctg cacgagaagc
     4141 agaaaaccgg aggcgcgccg tcgacctcca tcacggccta taagagtgag ggggagtcag
     4201 cggagttctc cttcccactc aaccttggag aggaaagcct gcagggagag ttgagatgga
     4261 aggcagagaa ggctccttct tcccagtcct ggatcacctt ctccctaaag aaccaaaagg
     4321 tgtctgtgca gaagtctact agcaacccca agttccagct gtccgaaacg ctcccactca
     4381 cccttcagat accccaggtc tcccttcagt ttgctggttc tggcaacctg accctgactc
     4441 tggacagagg gatactgtat caggaagtga acctggtggt gatgaaagtg actcagcccg
     4501 acagcaacac tttgacctgt gaggtgatgg gacccacctc acccaagatg agactgatct
     4561 tgaagcagga gaatcaggag gccagggtct ccaggcagga gaaagtgatt caagtgcagg
     4621 cccctgaagc aggggtgtgg caatgtctac tgagtgaagg tgaagaggtc aagatggact
     4681 ccaagatcca ggttttatcc aaagggttga attccggatc actgcatcat attctggatg
     4741 cacagaaaat ggtgtggaat catcgttaag tctagacacg tgattaatta aggatccccc
     4801 gacctcgacc tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt
     4861 tttgtgtctc tcactcggaa ggacatatgg gagggcaaat catttggtcg agatccctcg
     4921 gagatctcta gctagaggat cgatccccgc cccggacgaa ctaaacctga ctacgacatc
     4981 tctgcccctt cttcgcgggg cagtgcatgt aatcccttca gttggttggt acaacttgcc
     5041 aactgggccc tgttccacat gtgacacggg gggggaccaa acacaaaggg gttctctgac
     5101 tgtagttgac atccttataa atggatgtgc acatttgcca acactgagtg gctttcatcc
     5161 tggagcagac tttgcagtct gtggactgca acacaacatt gcctttatgt gtaactcttg
     5221 gctgaagctc ttacaccaat gctgggggac atgtacctcc caggggccca ggaagactac
     5281 gggaggctac accaacgtca atcagagggg cctgtgtagc taccgataag cggaccctca
     5341 agagggcatt agcaatagtg tttataaggc ccccttgtta accctaaacg ggtagcatat
     5401 gcttcccggg tagtagtata tactatccag actaacccta attcaatagc atatgttacc
     5461 caacgggaag catatgctat cgaattaggg ttagtaaaag ggtcctaagg aacagcgata
     5521 tctcccaccc catgagctgt cacggtttta tttacatggg gtcaggattc cacgagggta
     5581 gtgaaccatt ttagtcacaa gggcagtggc tgaagatcaa ggagcgggca gtgaactctc
     5641 ctgaatcttc gcctgcttct tcattctcct tcgtttagct aatagaataa ctgctgagtt
     5701 gtgaacagta aggtgtatgt gaggtgctcg aaaacaaggt ttcaggtgac gcccccagaa
     5761 taaaatttgg acggggggtt cagtggtggc attgtgctat gacaccaata taaccctcac
     5821 aaaccccttg ggcaataaat actagtgtag gaatgaaaca ttctgaatat ctttaacaat
     5881 agaaatccat ggggtgggga caagccgtaa agactggatg tccatctcac acgaatttat
     5941 ggctatgggc aacacataat cctagtgcaa tatgatactg gggttattaa gatgtgtccc
     6001 aggcagggac caagacaggt gaaccatgtt gttacactct atttgtaaca aggggaaaga
     6061 gagtggacgc cgacagcagc ggactccact ggttgtctct aacacccccg aaaattaaac
     6121 ggggctccac gccaatgggg cccataaaca aagacaagtg gccactcttt tttttgaaat
     6181 tgtggagtgg gggcacgcgt cagcccccac acgccgccct gcggttttgg actgtaaaat
     6241 aagggtgtaa taacttggct gattgtaacc ccgctaacca ctgcggtcaa accacttgcc
     6301 cacaaaacca ctaatggcac cccggggaat acctgcataa gtaggtgggc gggccaagat
     6361 aggggcgcga ttgctgcgat ctggaggaca aattacacac acttgcgcct gagcgccaag
     6421 cacagggttg ttggtcctca tattcacgag gtcgctgaga gcacggtggg ctaatgttgc
     6481 catgggtagc atatactacc caaatatctg gatagcatat gctatcctaa tctatatctg
     6541 ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg
     6601 ggtagtatat gctatcctaa tttatatctg ggtagcatag gctatcctaa tctatatctg
     6661 ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa tctgtatccg
     6721 ggtagcatat gctatcctaa tagagattag ggtagtatat gctatcctaa tttatatctg
     6781 ggtagcatat actacccaaa tatctggata gcatatgcta tcctaatcta tatctgggta
     6841 gcatatgcta tcctaatcta tatctgggta gcataggcta tcctaatcta tatctgggta
     6901 gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaattta tatctgggta
     6961 gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     7021 gtatatgcta tcctaatctg tatccgggta gcatatgcta tcctcatgat aagctgtcaa
     7081 acatgagaat tttcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     7141 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     7201 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     7261 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     7321 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     7381 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     7441 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     7501 agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag
     7561 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     7621 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     7681 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     7741 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     7801 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg
     7861 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     7921 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     7981 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     8041 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     8101 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     8161 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     8221 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     8281 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     8341 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     8401 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     8461 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     8521 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     8581 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     8641 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     8701 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     8761 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     8821 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     8881 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     8941 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     9001 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     9061 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     9121 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     9181 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     9241 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     9301 cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt cgaccaattc
     9361 tcatgtttga cagcttatca tcgcagatcc gggcaacgtt gttgccattg ctgcaggcgc
     9421 agaactggta ggtatgga
//
