LOCUS       sequence-68916-e         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expesses the human codon usage Cas9 nuclease (Mali et al. Science 
            339, 823-826, 2013) with an N-terminal GFP tag from the 35S promoter
            in the plant tissue .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Nekrasov V, Staskawicz B, Weigel D, Jones JD, Kamoun S
  TITLE     Targeted mutagenesis in the model plant Nicotiana benthamiana using 
            Cas9 RNA-guided endonuclease.
  JOURNAL   Nat Biotechnol. 2013 Aug 8;31(8):691-3. doi: 10.1038/nbt.2655.
  PUBMED    23929340
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt"; 
            journalName: "Nat Biotechnol"; date: "2013-08-8- 8"; volume: "31"; 
            issue: "8"; pages: "691-3"
COMMENT     Sequence Label: pK7WGF2::hCas9
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(63..242)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     protein_bind    614..638
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(657..677)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 actgcgtgca atccatcttg ttcaatcatg cctcgatcga gttgagagtg aatatgagac
       61 tctaattgga taccgagggg aatttatgga acgtcagtgg agcatttttg acaagaaata
      121 tttgctagct gatagtgacc ttaggcgact tttgaacgcg caataatggt ttctgacgta
      181 tgtgcttagc tcattaaact ccagaaaccc gcggctcagt ggctccttca acgttgcggt
      241 tctgtcagtt ccaaacgtaa aacggcttgt cccgcgtcat cggcgggggt cataacgtga
      301 ctcccttaat tctccgctca tgatcgataa ttcgcggtac ccggggatcc tctagagggc
      361 ccgacgtcgc atgcctgcag gtcactggat tttggtttta ggaattagaa attttattga
      421 tagaagtatt ttacaaatac aaatacatac taagggtttc ttatatgctc aacacatgag
      481 cgaaacccta taagaaccct aattccctta tctgggaact actcacacat tattctggag
      541 aaaaatagag agagatagat ttgtagagag agactggtga tttttgcgga ctctagcatg
      601 gccgcgggat atcaccactt tgtacaagaa agctgggtcg gcgcgcccac ccttcacacc
      661 ttcctcttct tcttggggtc agccctgctg tctccaccga gctgagagag gtcgattctt
      721 gtttcataga gccccgtaat tgactgatga atcagtgtgg cgtccaggac ctcctttgta
      781 gaggtgtacc gctttctgtc tatggtggtg tcgaagtact tgaaggctgc aggcgcgccc
      841 aagttggtca gagtaaacaa gtggataatg ttttctgcct gc
//
