LOCUS       sequence-68918-e         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Frokjaer-Jensen C, Davis MW, Ailion M, Jorgensen EM
  TITLE     Improved Mos1-mediated transgenesis in C. elegans.
  JOURNAL   Nat Methods. 2012 Jan 30;9(2):117-8. doi: 10.1038/nmeth.1865.
  PUBMED    22290181
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-01-30- 30"; volume: "9"; 
            issue: "2"; pages: "117-8"
COMMENT     Sequence Label: pCFJ352 - MCS(ttTi4348, I)
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(167..183)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(167..183)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    complement(190..214)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tagagggtac cagagctcac ctaggtatct agaaccggtg acgtcaccat gggaagcttc
       61 gtggatccag atatcctgca ggaattcctc gagacgtacg gtgcgcgcga tgcattcgaa
      121 gatctgccca ctagtgagtc gtattacgta gcttggcgta atcatggtca tagctgtttc
      181 ctgcccgtga cccagctttc ttgtacaaag tggcagtaat caattatttt tcgctccccc
      241 ctcctcgcaa gtcggcgatt tctttgaagt tttgtcaaaa gaagagacaa ggacttgata
      301 gatacgctca tcagagtgag attcattgca aatagtgtgt aaatcgtgga gagacgagag
      361 aaaaggtgtg agaagcttag aagataaaga ataggaagaa gaacatatct catttcctat
      421 tttctctaat ttttcgttca tttttctaga cattacccat ttgatggttt ttcaatatgg
      481 ttatcagggt actcattttg gtagttttca aagcaatttg gagttttttt ttaaattgtc
      541 aactacaaaa acgcagctta aaggtccaaa tatcatagta taaaaattct ttaaaaaatt
      601 ccaaatttta tatgattttt tgaaaattgg aagaatgtta gtttttgcca tttttttgga
      661 agtttttctc cggattttat ggtacttata tttcaaatat aaatttttgt aggaattcga
      721 gtatgcgaga gagctatagt ttactaaaaa aaagtggcaa aaactcagta attgccactt
      781 ttttcaaaaa tgttttgaaa aattttacta tgatatttgg ccattttggc accatatgta
      841 cgttact
//
