LOCUS       sequence-68965-e         922 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 D10A+H840A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Mali P, Aach J, Stranges PB, Esvelt KM, Moosburner M, Kosuri S, Yang
            L, Church GM
  TITLE     CAS9 transcriptional activators for target specificity screening and
            paired nickases for cooperative genome engineering.
  JOURNAL   Nat Biotechnol. 2013 Aug 1. doi: 10.1038/nbt.2675.
  PUBMED    23907171
REFERENCE   2  (bases 1 to 922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2013 Aug 1. doi: 10.1038/nbt.2675."
COMMENT     Sequence Label: Cas9m2
FEATURES             Location/Qualifiers
     source          1..922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      65..74
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ttttgacctc catagaagac accgggaccg atccagcctc cggactctag aggatcgaac
       61 ccttgccacc atggacaaga agtactccat tgggctcgct atcggcacaa acagcgtcgg
      121 ctgggccgtc attacggacg agtacaaggt gccgagcaaa aaattcaaag ttctgggcaa
      181 taccgatcgc cacagcataa agaagaacct cattggcgcc ctcctgttcg actccgggga
      241 gacggccgaa gccacgcggc tcaaaagaac agcacggcgc agatataccc gcagaaagaa
      301 tcggatctgc tacctgcagg agatctttag taatgagatg gctaaggtgg atgactcttt
      361 cttccatagg ctggaggagt cctttttggt ggaggaggat aaaaagcacg agcgccaccc
      421 aatctttggc aatatcgtgg acgaggtggc gtaccatgaa aagtacccaa ccatatatca
      481 tctgaggaag aagcttgtag acagtactga taaggctgac ttgcggttga tctatctcgc
      541 gctggcgcat atgatcaaat ttcggggaca cttcctcatc gagggggacc tgaacccaga
      601 caacagcgat gtcgacaaac tctttatcca actggttcag acttacaatc agcttttcga
      661 agagaacccg atcaacgcat ccggagttga cgccaaagca atcctgagcg ctaggctgtc
      721 caaatcccgg cggctcgaaa acctcatcgc acagctccct ggggagaaga agaacggcct
      781 gtttggtaat cttatcgccc tgtcactcgg gctgaccccc aactttaaat ctaacttcga
      841 cctggccgaa gatgccaagc ttcaactgag caaagacacc tacgatgatg atctcgacaa
      901 tctgctggcc cagatcggcg ac
//
