LOCUS       sequence-69081-e         885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Katoh Y, Ritter B, Gaffry T, Blondeau F, Honing S, McPherson PS
  TITLE     The clavesin family, neuron-specific lipid- and clathrin-binding 
            Sec14 proteins regulating lysosomal morphology.
  JOURNAL   J Biol Chem. 2009 Oct 2;284(40):27646-54. doi: 
            10.1074/jbc.M109.034884. Epub 2009 Aug 3.
  PUBMED    19651769
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M109.034884"; journalName: "J Biol Chem"; date: 
            "2009-10-2- 2"; volume: "284"; issue: "40"; pages: "27646-54"
COMMENT     Sequence Label: Flag-Tagged Clavesin1 (pCMV-Tag2B-Sec14a)
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        40..58
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      93..102
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             102..125
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gtgaaccgtc agatccgcta gcgattacgc caagctcgaa attaaccctc actaaaggga
       61 acaaaagctg gagctccacc gcggtggcgg ccgccaccat ggattacaag gatgacgacg
      121 ataagagccc gggcggatcc atgggaccag tctctcttct tccaaaatat cagaagttaa
      181 acacttggaa cggagatttg gccaagatga cccatttaca ggctggactc agtccagaga
      241 ctatagagaa agctcgcctg gaactgaatg aaaaccccga tgttttacat caggatattc
      301 agcaagtcag ggacatgatc atcaccaggc ctgacattgg atttttacgt acagatgatg
      361 ccttcatcct gagatttctc cgagccagga agtttcacca agcggatgcc tttagactcc
      421 tggctcagta tttccagtac cgccagctaa acctggacat gttcaaaaac ttcaaggcag
      481 atgatcccgg cattaagagg gctctgatcg atgggttccc cggggtgctg gaaaaccgag
      541 accattacgg caggaagatt cttttgctgt ttgcagccaa ttgggatcag agtaggaact
      601 ccttcacaga catccttcgt gccatcctgc tgtcattgga agtcctaatc gaagatccgg
      661 agcttcagat aaatggcttc attttaatta tagactggag taatttttcc ttcaaacaag
      721 cctccaaact gacaccttca atccttaaac tggccattga agggttgcag gacagctttc
      781 ctgcccgctt tggaggagtc cactttgtca accagccctg gtacattcat gccctctaca
      841 cactcatcaa gccatttctt aaagacaaga ccaggaaacg gattt
//
