LOCUS       sequence-69186-e         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for expression of GFP-FLAG tagged human XRCC4 isoform 2 
            (N-terminal tag). Confers resistance to G418..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Britton S, Coates J, Jackson SP
  TITLE     A new method for high-resolution imaging of Ku foci to decipher 
            mechanisms of DNA double-strand break repair.
  JOURNAL   J Cell Biol. 2013 Jul 29.
  PUBMED    23897892
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2013 Jul 29."
COMMENT     Sequence Label: pEGFP-C1-FLAG-XRCC4
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             28..51
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             58..81
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gagctgtaca agtccggact cagatctgat tacaaggatg acgacgataa gctcgaggac
       61 tacaaggacg atgacgacaa gggatccgga atggagagaa aaataagcag aatccacctt
      121 gtttctgaac ccagtataac tcattttcta caagtatctt gggagaaaac actggaatct
      181 ggttttgtta ttacacttac tgatggtcat tcagcatgga ctgggacagt ttctgaatca
      241 gagatttccc aagaagctga tgacatggca atggaaaaag ggaaatatgt tggtgaactg
      301 agaaaagcat tgttgtcagg agcaggacca gctgatgtat acacgtttaa tttttctaaa
      361 gagtcttgtt atttcttctt tgagaaaaac ctgaaagatg tctcattcag acttggttcc
      421 ttcaacctag agaaagttga aaacccagct gaagtcatta gagaacttat ttgttattgc
      481 ttggacacca ttgcagaaaa tcaagccaaa aatgagcacc tgcagaaaga aaatgaaagg
      541 cttctgagag attggaatga tgttcaagga cgatttgaaa aatgtgtgag tgctaaggaa
      601 gctttggaga ctgatcttta taagcggttt attctggtgt tgaatgagaa gaaaacaaaa
      661 atcagaagtt tgcataataa attattaaat gcagctcaag aacgagaaaa ggacatcaaa
      721 caagaagggg aaactgcaat ctgttctgaa atgactgctg accgggaccc cgtgtacgac
      781 gagagtactg atgaggaaag tgaaaaccaa actgatctct ctgggttggc ttcagctgct
      841 gtaagtaaag atgattccat tatttcaagt cttgatgtca ctga
//
