LOCUS       sequence-69264-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  triple mutation used for co-IP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Huang N, Chelliah Y, Shan Y, Taylor CA, Yoo SH, Partch C, Green CB, 
            Zhang H, Takahashi JS
  TITLE     Crystal structure of the heterodimeric CLOCK:BMAL1 transcriptional 
            activator complex.
  JOURNAL   Science. 2012 Jul 13;337(6091):189-94. doi: 10.1126/science.1222804.
            Epub 2012 May 31.
  PUBMED    22653727
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1222804"; journalName: "Science"; date: 
            "2012-07-13- 13"; volume: "337"; issue: "6091"; pages: "189-94"
COMMENT     Sequence Label: pCMV10/3xflag-Clock L57E/L113E/W284A
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             21..86
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 aaccgtcaga attaaccatg gactacaaag accatgacgg tgattataaa gatcatgaca
       61 tcgattacaa ggatgacgat gacaagcttg cggccgcgat ggtgtttacc gtaagctgta
      121 gtaaaatgag ctcaattgtt gacagagatg acagtagtat ttttgatgga ttggtggaag
      181 aagatgacaa ggacaaagca aaaagagtat ctagaaacaa atcagaaaag aaacgtagag
      241 atcagttcaa tgtcctcatt aaggaggagg ggtctatgct tcctggtaac gcgagaaaga
      301 tggacaagtc tactgttcta cagaagagca ttgatttttt gcgcaaacat aaagagacca
      361 ctgcacagtc agatgctagt gagattcgac aggactggaa acccacattc cttagtaatg
      421 aagagtttac acaggagatg ttagaggctc ttgatggttt ttttttagcg atcatgacag
      481 atggaagtat aatatatgta tctgagagtg taacttcgtt acttgaacat ttaccatctg
      541 atcttgtgga tcaaagtata tttaatttta tcccagaggg agaacattca gaggtttata
      601 agatactctc tactcatctg ctggaaagtg actcattaac ccctgagtac ttaaaatcaa
      661 aaaatcagtt agaattctgt tgtcacatgc ttcgaggaac aatagaccca aaggagccat
      721 ccacctatga atatgtgaga tttataggaa attttaaatc tttaaccagt gtatcaactt
      781 caacacacaa tggttttgaa ggaactatac aacgcacaca taggccttct tatgaagata
      841 gagtttgttt tgtagctact gtcagattag ctacacctca
//
