LOCUS       sequence-69270-e         962 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses I-Sce I nuclease and BFP tracker in mammalian cells, codes
            for eGFP donor sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Kuhar R, Gwiazda KS, Humbert O, Mandt T, Pangallo J, Brault M, Khan 
            I, Maizels N, Rawlings DJ, Scharenberg AM, Certo MT
  TITLE     Novel fluorescent genome editing reporters for monitoring DNA repair
            pathway utilization at endonuclease-induced breaks.
  JOURNAL   Nucleic Acids Res. 2013 Oct 10.
  PUBMED    24121685
REFERENCE   2  (bases 1 to 962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2013 Oct 10."
COMMENT     Sequence Label: pEX.d14GFP.sEF1a.Kozak.HA.NLS.Sce.T2A.BFP
FEATURES             Location/Qualifiers
     source          1..962
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     227..248
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     464..483
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        558..662
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(881..900)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gtgcgctcct ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg
       61 tggtcggggt agcggctgaa gcactgcacg ccgtaggtca gggtggtcac gagggtgggc
      121 cagggcacgg gcagcttgcc ggtggtgcag atgaacttca gggtcagctt gccgtaggtg
      181 gcatcgccct cgccctcgcc ggacacgctg aacttgtggc cgtttacgtc gccgtccagc
      241 tcgaccagga tgggcaccac cccggtgaac agctcctcgc ccttgctcac catacgcgta
      301 tatctggccc gtacatcgcg aagcagcgca aaacgcctaa ccctaagcag attcttcatg
      361 caattgtcgg tcaagccttg ccttgttgta gcttaaattt tgctcgcgca ctactcagcg
      421 acctccaaca cacaagcagg gagcagatac tggcttaact atgcggcatc agagcagatt
      481 gtactgagag tgcaccatag gggatcggga gatctcccga tccgtcgacg tcaggtggca
      541 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
      601 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
      661 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
      721 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
      781 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
      841 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
      901 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
      961 tg
//
