LOCUS       sequence-69315-e        1029 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses I-Sce I nuclease and BFP tracker in mammalian cells, codes
            for eGFP donor sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Kuhar R, Gwiazda KS, Humbert O, Mandt T, Pangallo J, Brault M, Khan 
            I, Maizels N, Rawlings DJ, Scharenberg AM, Certo MT
  TITLE     Novel fluorescent genome editing reporters for monitoring DNA repair
            pathway utilization at endonuclease-induced breaks.
  JOURNAL   Nucleic Acids Res. 2013 Oct 10.
  PUBMED    24121685
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2013 Oct 10."
COMMENT     Sequence Label: pEX.d14GFP.sEF1a.Kozak.HA.NLS.Sce.T2A.BFP
FEATURES             Location/Qualifiers
     source          1..1029
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(54..755)
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             complement(756..809)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 cagtcaatct ttcacaaatt ttgtaatcca gaggttgatt gtcgactcta gaatcaatta
       61 agcttgtgcc ccagtttgct agggaggtcg cagtatctgg ccactgccac ctcgtgctgc
      121 tcgacgtagg tctcgttgtt ggcctccttg attctttcca gtctgtagtc cacatagtag
      181 acgccaggca tcttgaggtt cttagcgggt ttcttggatc tatatgtggt cttgatgttt
      241 gcgatcagat ggctcccacc cacgagcttc agggccatgt cgtttctgcc ttccaggccg
      301 ccgtcagcgg ggtacagcgt ctcggtgaag gcctcccagc cgagtgtttt cttctgcatc
      361 acagggccgt tggatgtgaa gttcacccct ctgatcttga cgttgtagat gaggcagccg
      421 tcctggaggc tggtgtcctg ggtagcggtc agcacgcccc cgtcttcgta tgtggtgact
      481 ctctcccatg tgaagccctc agggaaggac tgcttgaaga agtcggggat gccctgggtg
      541 tggttgatga aggtcttgct gccgtagagg aagctagtag ccaggatgtc gaaggcgaag
      601 gggagagggc cgccctcgac caccttgatt ctcatggtct gggtgccctc gtagggcttg
      661 ccttcgccct cggatgtgca cttgaagtga tggttgtcca cggtgccctc catgtacagc
      721 ttcatgtgca tgttctcctt aatcagctcg ctcatggggc ccggattctc ctccacgtca
      781 ccgcatgtta gaagacttcc tctgccctca ccggatccgc cgccgccctt caggaaggtc
      841 tcgctgctga tggtgttggg cagcttgtac atcatctggg ggatcaggta gggcttgatc
      901 aggttgtaga agatcaggta gctcatgctg tcgatgtaga tgatgggctt gttcttgttg
      961 atcttcacgt agcagttcag ctggaacttg ttcctcaggc ccttcaccag gtactccacc
     1021 tcctcgaag
//
