LOCUS       sequence-69451-e         807 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bmal fragment cloned with Venus tag used for mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Huang N, Chelliah Y, Shan Y, Taylor CA, Yoo SH, Partch C, Green CB, 
            Zhang H, Takahashi JS
  TITLE     Crystal structure of the heterodimeric CLOCK:BMAL1 transcriptional 
            activator complex.
  JOURNAL   Science. 2012 Jul 13;337(6091):189-94. doi: 10.1126/science.1222804.
            Epub 2012 May 31.
  PUBMED    22653727
REFERENCE   2  (bases 1 to 807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1222804"; journalName: "Science"; date: 
            "2012-07-13- 13"; volume: "337"; issue: "6091"; pages: "189-94"
COMMENT     Sequence Label: HLH Bmal Pas 1-465-VenC I317D
FEATURES             Location/Qualifiers
     source          1..807
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(153..404)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(195..216)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      407..416
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
       61 ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat cagttatcta
      121 gatccggtgg atcccgaggg cagcattccg gacttgtaca gctcgtccat gccgagagtg
      181 atcccggcgg cggtcacgaa ctccagcagg accatgtgat cgcgcttctc gttggggtct
      241 ttgctcaggg cggactggta gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg
      301 ccgatggggg tgttctgctg gtagtggtcg gcgagctgca cgccgccgtc ctcgatgttg
      361 tggcggatct tgaagttggc cttgatgccg ttcttctgct tgtccaccat ggtggcgacc
      421 gggctgtcca tgctgtgggg gggtgctgtt agctgcggga aggttgggtc cccgccttcc
      481 aggacattgg ctaaaacaac agtgttggtt gagacaatgt attcaacttc cttggtccac
      541 gggttcatga aactgaacca tcgacttcgt agcgtgataa aagaaccatc tttgatctta
      601 aacttatagc aattagttgt gatcttttct cttgtctgta aaacttgcct gtgacattct
      661 gcgaggtgtc ctatgtcgtc ttgatgaaaa tactcataac atgatgtacc tagaagttcc
      721 tgtggtagat acgccaaaat agctgtcgcc ctctgatcta caaaaacaaa tttcccatct
      781 attgcgtgtc gagaaacgta ctccata
//
