LOCUS       sequence-69634-e        1032 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  engineered receptor improves heteromeric receptor expression and 
            sensitivity to IVM..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Frazier SJ, Cohen BN, Lester HA
  TITLE     An Engineered GluCl Channel for Sensitive, Consistent Neuronal 
            Silencing by Ivermectin.
  JOURNAL   J Biol Chem. 2013 May 29.
  PUBMED    23720773
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2013 May 29."
COMMENT     Sequence Label: pcDNA3.1 V5 His-TOPO-GluCl optbeta-m-YFP Y182F...
FEATURES             Location/Qualifiers
     source          1..1032
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      152..161
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tggctagtta agcttggtac cgagctcgga
      121 tccactagtc cagtgtggtg gaattgccct tgccaccatg gccaccccct ccagcttctc
      181 gatcctgctg ctgctgctgc tgatgcccgt ggtgaccaac ggcgagtaca gcatgcagag
      241 cgagcaggag atcctgaatg ccctgctgaa gaactacgac atgcgcgtgc gccccccccc
      301 cgccaacagc agcaccgagg gcgccgtgaa cgtgagagtg aacatcatga tcagaatgct
      361 gagcaagatc gacgtggtga acatggagta ctccatccag ctgaccttcc gcgagcagtg
      421 gatcgacccc cgcctggcct acgagaacct gggcttctac aacccccccg ccttcctgac
      481 cgtgccccac gtcaagaagt ccctgtggat acccgacacc ttcttcccca ccgagaaggc
      541 cgcccaccgc cacctgatcg acatggagaa catgttcctg cgcatctacc ccgacggcaa
      601 gatcctgtac agcagccgca tcagcctgac gagctcctgc cccatgcgcc tgcagctgta
      661 ccccctggac taccagagct gcaacttcga cctggtgtcc ttcgcccaca ccatgaacga
      721 catcatgtat gagtgggacc ccagcacccc cgtgcagctg aagcccggcg tgggctccga
      781 cctgcccaac ttcatcctga agaactacac caccaacgcc gactgcacct cccacaccaa
      841 cactggctcg tacggctgcc tgcgcatgca gctgctgttc aagcgccagt tcagctacta
      901 cctggtgcag ctgtacgccc ccaccaccat gatcgtgatc gtgagctggg tcagcttctg
      961 gatcgacctg cacagcaccg ccggccgcgt ggccctgggc gtgaccaccc tgctgaccat
     1021 gaccaccatg ca
//
