LOCUS       sequence-69643-e        1050 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Codes for the SSA-TLR with the target site for the CCR5 TALEN with 
            the spacer region corresponding to I-Sce I in a lentiviral backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Kuhar R, Gwiazda KS, Humbert O, Mandt T, Pangallo J, Brault M, Khan 
            I, Maizels N, Rawlings DJ, Scharenberg AM, Certo MT
  TITLE     Novel fluorescent genome editing reporters for monitoring DNA repair
            pathway utilization at endonuclease-induced breaks.
  JOURNAL   Nucleic Acids Res. 2013 Oct 10.
  PUBMED    24121685
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2013 Oct 10."
COMMENT     Sequence Label: pCVL.SSA TLR (CCR5 TALEN Sce Spacer)
FEATURES             Location/Qualifiers
     source          1..1050
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      51..60
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             900..953
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(996..1017)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ccctcactcg gcgcgccagt cctccgacag actgagtcgc ccgctcgagc gccaccatgg
       61 ctgaaggatc cgtcgccagg cagcctgacc tcttgacctg cgacgatgag ccgatccata
      121 tccccggtgc catccaaccg catggactgc tgctcgccct cgccgccgac atgacgatcg
      181 ttgccggcag cgacaacctt cccgaactca ccggactggc gatcggcgcc ctgatcggcc
      241 gctctgcggc cgatgtcttc gactcggaga cgcacaaccg tctgacgatc gccttggccg
      301 agcccggggc ggccgtcgga gcaccgatca ctgtcggctt cacgatgcga aaggacgcag
      361 gcttcatcgg ctcctggcat cgccatgatc agctcatctt cctcgagctc gagcctcccc
      421 agcgggacgt cgccgagccg caggcgttct tccgccgcac caacagcgcc atccgccgcc
      481 tgcaggccgc cgaaaccttg gaaagcgcct gcgccgccgc ggcgcaagag gtgcggaaga
      541 ttaccggctt cgatcgggtg atgatctatc gcttcgcctc cgacttcagc ggcgaagtga
      601 tcgcagagga tcggtgcgcc gaggtcgagt caaaactagg cctgcactat cctgcctcaa
      661 ccgtgccggc gcaggcccgt cggctctata ccatcaaccc ggtacggatc attcccgata
      721 tcaattatcg gccggtgccg gtcaccccag acctcaatcc ggtcaccggg cggccgattg
      781 atcttagctt cgccatcctg cgcagcgtct cgcccgtcca tctggaattc atgcgcaaca
      841 taggcatgca cggcacgatg tcgatctcga ttttgcgcgg cgagcgactg tggtctagag
      901 agggcagagg aagtcttcta acatgcggtg acgtggagga gaatccgggc cccgtgagca
      961 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     1021 acggccacaa gttcagcgtg tccggcgagg
//
