LOCUS       sequence-69676-e         792 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  used to create transgenic mice expressing LacZ reporter with Nestin 
            enhancer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Lothian C, Prakash N, Lendahl U, Wahlstrom GM
  TITLE     Identification of both general and region-specific embryonic CNS 
            enhancer elements in the nestin promoter.
  JOURNAL   Exp Cell Res. 1999 May 1;248(2):509-19.
  PUBMED    10222142
REFERENCE   2  (bases 1 to 792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Exp Cell 
            Res."; date: "1999-05-1"; volume: "248(2)"; pages: "509-19"
COMMENT     Sequence Label: nes374tk/lacZ
FEATURES             Location/Qualifiers
     source          1..792
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     186..208
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     200..217
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     201..217
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        712..774
                     /label=Mini-TK promoter
                     /note="minimal herpes simplex virus (HSV) thymidine kinase 
                     promoter"
ORIGIN
        1 caccatatgc ggtgtgaaat accgcacaga tgcgtaaggg agaaaatacc gcatcaggcg
       61 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
      121 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
      181 gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc aagcttgcat gcctgcaggt
      241 cgacctacac acacccacac aaggcttagg ctagcctcct cctcccaccc caggccaatg
      301 acctcatcct ctgtgtgggg gaccgattct catgctaatt gctgccattg tccaagccac
      361 catctggagg gtctgagaag gagaggattc tggggaagtg ggaattctca ggccgttccc
      421 atgggatggg ctccctcctc cttccccggt ggcccccaga gctagcgaca gacaaaagca
      481 aatgaattca gctccccctc tccaaatcct tttcatgcct caaaaaatag cagttaaatt
      541 cctcggggcc caccagaccc ttcccctcgg tggcttcact gggggtcagg gccctcatct
      601 caggatctct aagcagtcgt cgactctaga ggatccggcc ccgcccagcg tcttgtcatt
      661 ggcgaattcg aacacgcaga tgcagtcggg gcggcgcggt ccgaggtcca cttcgcatat
      721 taaggtgacg cgtgtggcct cgaacaccga gcgaccctgc agcgacccgc ttaacagcgt
      781 caacagcgtg cc
//
