LOCUS       sequence-69684-e         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  human gRNA expression vector targeting VEGF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Fu Y, Foden JA, Khayter C, Maeder ML, Reyon D, Joung JK, Sander JD
  TITLE     High-frequency off-target mutagenesis induced by CRISPR-Cas 
            nucleases in human cells.
  JOURNAL   Nat Biotechnol. 2013 Jun 23. doi: 10.1038/nbt.2623.
  PUBMED    23792628
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2013 Jun 23. doi: 10.1038/nbt.2623."
COMMENT     Sequence Label: pVC297 VEGF Site#1
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     121..140
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        219..294
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(478..497)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gttagagaga taattagaat taatttgact gtaaacacaa agatattagt acaaaatacg
       61 tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg
      121 gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg
      181 tggaaaggac gaaacaccgg gtggggggag tttgctccgt tttagagcta gaaatagcaa
      241 gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt
      301 taagcttggg ccgctcgagg tacctctcta catatgacat gtgagcaaaa ggccagcaaa
      361 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
      421 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
      481 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
      541 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
      601 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
      661 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
      721 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
      781 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
      841 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaa
//
