LOCUS       sequence-69692-e         950 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression of V5-tagged Luciferase in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Kang BH, Jensen KJ, Hatch JA, Janes KA
  TITLE     Simultaneous profiling of 194 distinct receptor transcripts in human
            cells.
  JOURNAL   Sci Signal. 2013 Aug 6;6(287):rs13. doi: 10.1126/scisignal.2003624.
  PUBMED    23921087
REFERENCE   2  (bases 1 to 950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2013-08-6"; volume: "6(287):rs13. doi"; pages: " 
            10.1126/scisignal.2003624"
COMMENT     Sequence Label: pLX302 Luciferase-V5 puro
FEATURES             Location/Qualifiers
     source          1..950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(68..109)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    111..135
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 caaattttgt aatccagagg ttgattgtcg acttaacgcg ccaccggtta gcgctagctc
       61 attactacgt agaatcgaga ccgaggagag ggttagggat aggcttacca accactttgt
      121 acaagaaagt tggcaacacg gcgatctttc cgcccttctt ggcctttatg aggatctctc
      181 tgatttttct tgcgtcgagt tttccggtaa gacctttcgg tacttcgtcc acaaacacaa
      241 ctcctccgcg caactttttc gcggttgtta cttgactggc gacgtaatcc acgatctctt
      301 tttccgtcat cgtctttccg tgctccaaaa caacaacggc ggcgggaagt tcaccggcgt
      361 catcgtcggg aagacctgcg acacctgcgt cgaagatgtt ggggtgttgg agcaagatgg
      421 attccaattc agcgggagcc acctgatagc ctttgtactt aatcagagac ttcaggcggt
      481 caacgatgaa gaagtgttcg tcttcgtccc agtaagctat gtctccagaa tgtagccatc
      541 catccttgtc aatcaaggcg ttggtcgctt ccggattgtt tacataaccg gacataatca
      601 taggacctct cacacacagt tcgcctcttt gattaacgcc cagcgttttc ccggtatcca
      661 gatccacaac cttcgcttca aaaaatggaa caactttacc gaccgcgccc ggtttatcat
      721 ccccctcggg tgtaatcaga atagctgatg tagtctcagt gagcccatat ccttgcctga
      781 tacctggcag atggaacctc ttggcaaccg cttccccgac ttccttagag aggggagcgc
      841 caccagaagc aatttcgtgt aaattagata aatcgtattt gtcaatcaga gtgcttttgg
      901 cgaagaagga gaatagggtt ggcaccagca gcgcactttg aatcttgtaa
//
