LOCUS       sequence-69716-e         776 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression plasmid for GST-3xHA-tagged constitutively 
            active MKK6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Bose AK, Janes KA
  TITLE     A high-throughput assay for phosphoprotein-specific phosphatase 
            activity in cellular extracts.
  JOURNAL   Mol Cell Proteomics. 2013 Mar;12(3):797-806. doi: 
            10.1074/mcp.O112.024059. Epub 2012 Dec 11.
  PUBMED    23233447
REFERENCE   2  (bases 1 to 776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/mcp.O112.024059"; journalName: "Mol Cell Proteomics"; date:
            "2013-03"; volume: "12"; issue: "3"; pages: "797-806"
COMMENT     Sequence Label: pGEX-4T-1 3xHA-MKK6-EE
FEATURES             Location/Qualifiers
     source          1..776
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             14..94
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
ORIGIN
        1 gcgtggatct atgtacccat acgatgttcc agattacgct tacccatacg atgttccaga
       61 ttacgcttac ccatacgatg ttccagatta cgctggatct atgtctcagt cgaaaggcaa
      121 gaagcgaaac cctggcctta aaattccaaa agaagcattt gaacaacctc agaccagttc
      181 cacaccacct agagatttag actccaaggc ttgcatttct attggaaatc agaactttga
      241 ggtgaaggca gatgacctgg agcctataat ggaactggga cgaggtgcgt acggggtggt
      301 ggagaagatg cggcacgtgc ccagcgggca gatcatggca gtgaagcgga tccgagccac
      361 agtaaatagc caggaacaga aacggctact gatggatttg gatatttcca tgaggacggt
      421 ggactgtcca ttcactgtca ccttttatgg cgcactgttt cgggagggtg atgtgtggat
      481 ctgcatggag ctcatggata catcactaga taaattctac aaacaagtta ttgataaagg
      541 ccagacaatt ccagaggaca tcttagggaa aatagcagtt tctattgtaa aagcattaga
      601 acatttacat agtaagctgt ctgtcattca cagagacgtc aagccttcta atgtactcat
      661 caatgctctc ggtcaagtga agatgtgcga ttttggaatc agtggctact tggtggacga
      721 ggttgctaaa gaaattgatg caggttgcaa accatacatg gcccctgaaa gaataa
//
