LOCUS       sequence-69747-e        1013 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BIOFAB RFP reporter plasmid for measuring promoter 14 + BCD5 
            efficiency..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Mutalik VK, Guimaraes JC, Cambray G, Lam C, Christoffersen MJ, Mai 
            QA, Tran AB, Paull M, Keasling JD, Arkin AP, Endy D
  TITLE     Precise and reliable gene expression via standard transcription and 
            translation initiation elements.
  JOURNAL   Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 
            2013 Mar 10.
  PUBMED    23474465
REFERENCE   2  (bases 1 to 1013)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2404"; journalName: "Nat Methods"; date: "2013-04"; 
            volume: "10"; issue: "4"; pages: "354-60"
COMMENT     Sequence Label: pFAB3909
FEATURES             Location/Qualifiers
     source          1..1013
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(350..355,356..372,373..379)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 acctgcgtgc aatccatctt gttcaatcat gcgaaacgat cctcatcctg tctcttgatc
       61 agatcatgat cccctgcgcc atcagatcct tggcggcaag aaagccatcc agtttacttt
      121 gcagggcttc ccaaccttac cagagggcgc cccagctggc aattccgacg tcgaattcaa
      181 aagatcttaa gtaagtaaga gtatacgtat atcggctaat aacgtattaa ggcgcttcgg
      241 cgcctttttt tatgggggta ttttcatccc aatccacacg tccaacgcac agcaaacacc
      301 acgtcgaccc tatcagctgc gtgctttcta tgagtcgttg ctgcataact tgacaattaa
      361 tcatccggct cgtataatgt gtggagggcc caagttcact taaaaaggag atcaacaatg
      421 aaagcaattt tcgtactgaa acatcttaat catgcagggg agggtttcta atggcgagta
      481 gcgaagacgt tatcaaagag ttcatgcgtt tcaaagttcg tatggaaggt tccgttaacg
      541 gtcacgagtt cgaaatcgaa ggtgaaggtg aaggtcgtcc gtacgaaggt acccagaccg
      601 ctaaactgaa agttaccaaa ggtggtccgc tgccgttcgc ttgggacatc ctgtccccgc
      661 agttccagta cggttccaaa gcttacgtta aacacccggc tgacatcccg gactacctga
      721 aactgtcctt cccggaaggt ttcaaatggg aacgtgttat gaacttcgaa gacggtggtg
      781 ttgttaccgt tacccaggac tcctccctgc aagacggtga gttcatctac aaagttaaac
      841 tgcgtggtac caacttcccg tccgacggtc cggttatgca gaaaaaaacc atgggttggg
      901 aagcttccac cgaacgtatg tacccggaag acggtgctct gaaaggtgaa atcaaaatgc
      961 gtctgaaact gaaagacggt ggtcactacg acgctgaagt taaaaccacc tac
//
