LOCUS       sequence-69754-e         938 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BIOFAB reporter plasmid for measuring T3 early termination 
            efficiency.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Mutalik VK, Guimaraes JC, Cambray G, Lam C, Christoffersen MJ, Mai 
            QA, Tran AB, Paull M, Keasling JD, Arkin AP, Endy D
  TITLE     Precise and reliable gene expression via standard transcription and 
            translation initiation elements.
  JOURNAL   Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 
            2013 Mar 10.
  PUBMED    23474465
REFERENCE   2  (bases 1 to 938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2404"; journalName: "Nat Methods"; date: "2013-04"; 
            volume: "10"; issue: "4"; pages: "354-60"
COMMENT     Sequence Label: pFAB804
FEATURES             Location/Qualifiers
     source          1..938
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             21..32
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      complement(149..178)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
ORIGIN
        1 tctcctttgc tcatggtacc tttctcctct ttaatgaatt gattaaccgg aagaaggtca
       61 atcataaagg ccactcttgc gaatgacctt gagtttgtcc ctctatagtg agtccgcctc
      121 tcctgttagt gagggttaat gcccggaacg aagaaaggcc cacccgtgaa ggtgagccag
      181 tgagttggtt acattttctc ttgagggtat cacataagta aagtgataat cataaaggcc
      241 actcgctagg agcgaccttg agtctatcac tcagcagatt attaagcacc ggtggagtga
      301 cgaccttcag cacgttcgta ctgttcaacg atggtgtagt cttcgttgtg ggaggtgatg
      361 tccagtttga tgtcggtttt gtaagcaccc ggcagctgaa ccggtttttt agccatgtag
      421 gtggttttaa cttcagcgtc gtagtgacca ccgtctttca gtttcagacg cattttgatt
      481 tcacctttca gagcaccgtc ttccgggtac atacgttcgg tggaagcttc ccaacccatg
      541 gtttttttct gcataaccgg accgtcggac gggaagttgg taccacgcag tttaactttg
      601 tagatgaact caccgtcttg cagggaggag tcctgggtaa cggtaacaac accaccgtct
      661 tcgaagttca taacacgttc ccatttgaaa ccttccggga aggacagttt caggtagtcc
      721 gggatgtcag ccgggtgttt aacgtaagct ttggaaccgt actggaactg cggggacagg
      781 atgtcccaag cgaacggcag cggaccacct ttggtaactt tcagtttagc ggtctgggta
      841 ccttcgtacg gacgaccttc accttcacct tcgatttcga actcgtgacc gttaacggaa
      901 ccttccatac gaactttgaa acgcatgaac tctttgat
//
