LOCUS       sequence-69789-e        3016 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3016)
  TITLE     Series-14: N-terminal Tag E.coli T7 Expression Vectors with Biobrick
            POLYPROMOTER Restriction Sites
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET E. coli expression vector with BioBrick
            po...
FEATURES             Location/Qualifiers
     source          1..3016
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      48..94
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      154..181
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        207..225
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      317..363
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      1530..1569
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      1600..2188
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2089..2108
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2342..2359
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2374..2513
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2439..2458)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2599..2621
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2615..2806)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
ORIGIN
        1 ggcaccatgc atcgatcccg cgaaatgcgg ccgcttaatt aaggcatcaa ataaaacgaa
       61 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg agtccttcca
      121 gtctgggcga aagcgcggtc gatcaaatta agcagaaggc catcctgacg gatggccttt
      181 ttgcgtttct atcgatcccg cgaaattaat acgactcact ataggcccct ctagaaataa
      241 ttttgtttaa ctttaagctt taagaaggag atatagatat cccaactcca taagtggctg
      301 ctgccaccgc tgagcatagc ataacccctt ggggcctcta aacgggtctt gaggggtttt
      361 ttgctgaaag gaggaactat atcaagtccc gaagtagctg tcagactgct ggcgaccgcc
      421 gcaactagtg gcgtcatgcg tgccgtatgg tctttcgcct gtagacgccc tacaagccgt
      481 actaaagtgg aaagacccgc aaaaggtatc gcgatcgccc tgcagggaac tatatccgac
      541 tagtagcccg cctaatgagc gggctttttt ttaattcccc tatttgttta tttttctaaa
      601 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt cgaaaaagga
      661 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
      721 ttcctgtttt tgctcaccca gaaacgctcg tgaaagtaaa agacgcagag gaccaattgg
      781 gggcacgagt gggatacata gaactggact tgaatagcgg taaaatcctt gagagttttc
      841 gccctgaaga gcgttttcca atgatgagca ctttcaaagt tctgctatgt ggagcagtat
      901 tatcccgtgt agatgcgggg caagagcaac tcggacgacg aatacactat tcgcagaatg
      961 acttggttga atactcccca gtgacagaaa agcaccttac ggacggaatg acggtaagag
     1021 aattatgtag tgccgccata acgatgagtg ataacactgc ggcgaactta cttctgacaa
     1081 ccatcggtgg accgaaggaa ttaaccgctt ttttgcacaa tatgggagac catgtaactc
     1141 gccttgaccg ttgggaacca gaactgaatg aagccatacc aaacgacgag cgagacacca
     1201 caatgcctgc ggcaatggca acaacattac gcaaactatt aactggcgaa ctacttactc
     1261 tggcttcacg gcaacaatta atagactggc ttgaagcgga taaagttgca ggaccactac
     1321 tgcgttcggc acttcctgct ggctggttta ttgctgataa atctggggca ggagagcgtg
     1381 gttcacgggg tatcattgcc gcacttggac cagatggtaa gccttcccgt atcgtagtta
     1441 tctacacgac gggtagtcag gcaactatgg acgaacgaaa tagacagatt gctgaaatag
     1501 gggcttcact gattaagcat tggtaaaccg atacaattaa aggctccttt tggagccttt
     1561 ttttttggac ggaccggtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     1621 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     1681 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     1741 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     1801 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     1861 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     1921 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     1981 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     2041 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     2101 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     2161 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     2221 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
     2281 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
     2341 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca
     2401 tctgtgcggt atttcacacc gcaatggtgc actctcagta caatctgctc tgatgccgca
     2461 tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac
     2521 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     2581 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     2641 aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt
     2701 ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc
     2761 tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt
     2821 aagggggatt tctgttcatg gggtaatgat accgatgaaa cgagagagga tgctcacgat
     2881 acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc
     2941 ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa
     3001 tacagatgta ggtgtt
//
